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PMID: 8332484 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An improved protocol for genomic sequencing and footprinting by ligation-mediated PCR.

Nucleic acids research ·Vol. 21 ·No. 11 ·1993-06-11 ·Pages 2779-81

Quivy JP, Becker PB

Abstract

暂无摘要

Related Genes
hsp27
MeSH Terms
Animals Base Sequence DNA/chemistry,genetics DNA-Directed DNA Polymerase Drosophila/genetics Genome Heat-Shock Proteins/genetics Indicators and Reagents Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction/methods
Chemicals
Heat-Shock Proteins Indicators and Reagents Oligodeoxyribonucleotides DNA DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Quivy J P
Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Becker P B
References (6)
6 references, click to expand
  1. Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions.
    Nucleic Acids Res. 1983 Nov 25;11(22):7853-71 PMID: 6359064
  2. Genomic sequencing and methylation analysis by ligation mediated PCR.
    Science. 1989 Nov 10;246(4931):810-3 PMID: 2814502
  3. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities.
    J Biol Chem. 1993 Jan 25;268(3):1965-75 PMID: 8420970
  4. Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.
    Nucleic Acids Res. 1992 Jul 25;20(14):3551-4 PMID: 1641322
  5. Extension product capture improves genomic sequencing and DNase I footprinting by ligation-mediated PCR.
    Nucleic Acids Res. 1992 Oct 25;20(20):5487-8 PMID: 1437574
  6. Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.
    Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1021-5 PMID: 1736283
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.11.2779
Published
1993-06-11
Pages
2779-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309631
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.11.2779 PMC Full Text View journal details
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