Home LiteratureArticle Details
PMID: 9548716 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Peroxisome biogenesis: involvement of ARF and coatomer.

The Journal of cell biology ·Vol. 141 ·No. 2 ·1998-04-20 ·Pages 373-83

Passreiter M, Anton M, Lay D, Frank R, Harter C, Wieland FT, Gorgas K, Just WW

Abstract

Peroxisomal membrane protein (Pmp)26p (RnPex11p), a major constituent of induced rat liver peroxisomal membrane, was found to contain a COOH-terminal, cytoplasmically exposed consensus dilysine motif with the potential to bind coatomer. Biochemical as well as immunocytochemical evidence is presented showing that peroxisomes incubated with preparations of bovine brain or rat liver cytosol recruit ADP-ribosylation factor (ARF) and coatomer in a strictly guanosine 5'-O-(3-thiotriphosphate)-dependent manner. Consistent with this observation, ldlF cells expressing a temperature-sensitive mutant version of the epsilon-subunit of coatomer exhibit elongated tubular peroxisomes possibly due to impaired vesiculation at the nonpermissive temperature. Since overexpression of Pex11p in Chinese hamster ovary wild-type cells causes proliferation of peroxisomes, these data suggest that Pex11p plays an important role in peroxisome biogenesis by supporting ARF- and coatomer-dependent vesiculation of the organelles.

MeSH Terms
ADP-Ribosylation Factors Amino Acid Sequence Animals Base Sequence Brain/metabolism CHO Cells Cattle Coatomer Protein Cricetinae Cytosol/metabolism Fungal Proteins/genetics,metabolism GTP-Binding Proteins/metabolism Glucosyltransferases/metabolism Golgi Apparatus/enzymology Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Intracellular Membranes/metabolism Liver/metabolism Male Membrane Proteins/genetics,metabolism Microbodies/enzymology,metabolism Molecular Sequence Data Peroxins Rats Rats, Wistar Transferases (Other Substituted Phosphate Groups)/metabolism
Chemicals
Coatomer Protein Fungal Proteins Membrane Proteins Peroxins Pex11a protein, rat Guanosine 5'-O-(3-Thiotriphosphate) Glucosyltransferases ceramide glucosyltransferase Transferases (Other Substituted Phosphate Groups) phosphatidylcholine-ceramide phosphocholine transferase GTP-Binding Proteins ADP-Ribosylation Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Passreiter M
Biochemie-Zentrum, Universität Heidelberg, D-69120 Heidelberg, Germany.
Anton M
Lay D
Frank R
Harter C
Wieland F T
Gorgas K
Just W W
References (68)
68 references, click to expand
  1. Nonclathrin coat protein gamma, a subunit of coatomer, binds to the cytoplasmic dilysine motif of membrane proteins of the early secretory pathway.
    Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1902-6 PMID: 8700856
  2. A unified nomenclature for peroxisome biogenesis factors.
    J Cell Biol. 1996 Oct;135(1):1-3 PMID: 8858157
  3. The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop.
    J Cell Biol. 1996 Apr;133(2):269-80 PMID: 8609161
  4. Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.
    EMBO J. 1996 Apr 15;15(8):1792-8 PMID: 8617224
  5. A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding.
    J Cell Biol. 1996 Dec;135(5):1239-48 PMID: 8947548
  6. Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6).
    J Biochem. 1996 Oct;120(4):813-9 PMID: 8947846
  7. Protein insertion into the peroxisomal membrane.
    Ann N Y Acad Sci. 1996 Dec 27;804:60-75 PMID: 8993536
  8. Regulated exocytosis in chromaffin cells. A potential role for a secretory granule-associated ARF6 protein.
    J Biol Chem. 1997 Jan 31;272(5):2788-93 PMID: 9006918
  9. The synthesis and turnover of rat liver peroxisomes. 3. The size distribution of peroxisomes and the incorporation of new catalase.
    J Cell Biol. 1970 May;45(2):408-15 PMID: 5513609
  10. The synthesis and turnover of rat liver peroxisomes. V. Intracellular pathway of catalase synthesis.
    J Cell Biol. 1973 Nov;59(2 Pt 1):507-24 PMID: 4805011
  11. Galloylglucoses of low molecular weight as mordant in electron microscopy. II. The moiety and functional groups possibly involved in the mordanting effect.
    J Cell Biol. 1976 Sep;70(3):622-33 PMID: 783173
  12. Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4344-8 PMID: 279920
  13. Biogenesis of peroxisomes: intracellular site of synthesis of catalase and uricase.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5066-70 PMID: 368807
  14. Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked oligosaccharides.
    J Biol Chem. 1979 Nov 25;254(22):11655-63 PMID: 500665
  15. Rat liver peroxisomes. I. New peroxisome population induced by thyroid hormones in the liver of male rats.
    Eur J Cell Biol. 1982 Feb;26(2):249-54 PMID: 6121707
  16. Microperoxisomes and peroxisomes in relation to lipid metabolism.
    Ann N Y Acad Sci. 1982;386:138-52 PMID: 6953844
  17. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  18. Membrane biogenesis: an overview.
    Methods Enzymol. 1983;96:xxix-lv PMID: 6656627
  19. Acyl-Coa oxidase and hydratase-dehydrogenase, two enzymes of the peroxisomal beta-oxidation system, are synthesized on free polysomes of clofibrate-treated rat liver.
    J Cell Biol. 1984 Dec;99(6):2241-6 PMID: 6501422
  20. Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7127-31 PMID: 6594687
  21. Improved isolation and purification of rat liver peroxisomes by combined rate zonal and equilibrium density centrifugation.
    Arch Biochem Biophys. 1985 Feb 15;237(1):124-34 PMID: 3970541
  22. Signal sequences. The limits of variation.
    J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
  23. Serial section analysis of mouse hepatic peroxisomes.
    Anat Embryol (Berl). 1985;172(1):21-32 PMID: 3898917
  24. Multiple cytosolic components promote intra-Golgi protein transport. Resolution of a protein acting at a late stage, prior to membrane fusion.
    J Biol Chem. 1986 Feb 15;261(5):2208-13 PMID: 3003102
  25. Biogenesis of peroxisomes.
    Annu Rev Cell Biol. 1985;1:489-530 PMID: 3916321
  26. Integral membrane polypeptides of rat liver peroxisomes: topology and response to different metabolic states.
    Arch Biochem Biophys. 1987 May 15;255(1):109-19 PMID: 3592654
  27. Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack.
    Cell. 1989 Jul 28;58(2):329-36 PMID: 2752426
  28. Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum.
    Cell. 1989 Aug 25;58(4):707-18 PMID: 2527615
  29. Clathrin, adaptors, and sorting.
    Annu Rev Cell Biol. 1990;6:151-71 PMID: 2177341
  30. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin.
    Cell. 1991 Feb 8;64(3):649-65 PMID: 1840503
  31. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein.
    Cell. 1991 Oct 18;67(2):239-53 PMID: 1680566
  32. Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targeting.
    Biochem Biophys Res Commun. 1991 Dec 31;181(3):947-54 PMID: 1764107
  33. Gene expression in hepatocyte-like lines established by targeted carcinogenesis in transgenic mice.
    Exp Cell Res. 1992 May;200(1):175-85 PMID: 1373387
  34. Peroxisomal protein import. In vivo evidence for a novel translocation competent compartment.
    FEBS Lett. 1992 Mar 30;300(2):179-82 PMID: 1563518
  35. Biogenesis of peroxisomes in mammals.
    Cell Biochem Funct. 1992 Sep;10(3):159-65 PMID: 1423896
  36. Gamma-COP, a coat subunit of non-clathrin-coated vesicles with homology to Sec21p.
    FEBS Lett. 1992 Dec 14;314(2):195-8 PMID: 1360908
  37. Membrane topology of the 22 kDa integral peroxisomal membrane protein.
    FEBS Lett. 1993 Jan 11;315(3):217-22 PMID: 8422909
  38. Comparative activity of ADP-ribosylation factor family members in the early steps of coated vesicle formation on rat liver Golgi membranes.
    J Biol Chem. 1997 Feb 14;272(7):4141-8 PMID: 9020126
  39. Coatomer (COPI)-coated vesicles: role in intracellular transport and protein sorting.
    Curr Opin Cell Biol. 1997 Aug;9(4):484-7 PMID: 9261053
  40. Cytosolic factors mediate protein insertion into the peroxisomal membrane.
    FEBS Lett. 1997 Sep 1;414(1):95-8 PMID: 9305739
  41. Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI.
    Cell. 1997 Sep 19;90(6):1137-48 PMID: 9323141
  42. Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane.
    EMBO J. 1997 Dec 15;16(24):7326-41 PMID: 9405362
  43. Import of firefly luciferase into peroxisomes of permeabilized Chinese hamster ovary cells: a model system to study peroxisomal protein import in vitro.
    Exp Cell Res. 1993 Mar;205(1):59-65 PMID: 8453998
  44. Import of firefly luciferase into mammalian peroxisomes in vivo requires nucleoside triphosphates.
    Exp Cell Res. 1993 Mar;205(1):66-75 PMID: 8453999
  45. Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
    J Biol Chem. 1993 Jun 5;268(16):12083-9 PMID: 8505331
  46. beta'-COP, a novel subunit of coatomer.
    EMBO J. 1993 Jul;12(7):2841-5 PMID: 8334999
  47. In vitro insertion of the 22-kD peroxisomal membrane protein into isolated rat liver peroxisomes.
    J Cell Biol. 1993 Dec;123(6 Pt 2):1717-25 PMID: 8276892
  48. Protein import into peroxisomes and biogenesis of the organelle.
    Annu Rev Cell Biol. 1993;9:445-78 PMID: 8280468
  49. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs.
    Science. 1994 Mar 18;263(5153):1629-31 PMID: 8128252
  50. Lactosylceramide is synthesized in the lumen of the Golgi apparatus.
    FEBS Lett. 1994 Mar 28;342(1):91-6 PMID: 8143857
  51. Disruptions in Golgi structure and membrane traffic in a conditional lethal mammalian cell mutant are corrected by epsilon-COP.
    J Cell Biol. 1994 Jun;125(6):1213-24 PMID: 8207054
  52. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
    Cell. 1994 Jun 17;77(6):895-907 PMID: 8004676
  53. Mechanisms of intracellular protein transport.
    Nature. 1994 Nov 3;372(6501):55-63 PMID: 7969419
  54. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.
    Cell. 1994 Dec 30;79(7):1199-207 PMID: 8001155
  55. Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p.
    J Cell Biol. 1995 Feb;128(4):509-23 PMID: 7860627
  56. Pmp27 promotes peroxisomal proliferation.
    J Cell Biol. 1995 Apr;129(2):345-55 PMID: 7721939
  57. Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth.
    Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3229-33 PMID: 7724544
  58. Structure and function of ARF proteins: activators of cholera toxin and critical components of intracellular vesicular transport processes.
    J Biol Chem. 1995 May 26;270(21):12327-30 PMID: 7759471
  59. Transmembrane helices predicted at 95% accuracy.
    Protein Sci. 1995 Mar;4(3):521-33 PMID: 7795533
  60. The emergence of clathrin-independent pinocytic pathways.
    Curr Opin Cell Biol. 1995 Aug;7(4):573-80 PMID: 7495578
  61. The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae.
    J Bacteriol. 1995 Dec;177(23):6773-81 PMID: 7592467
  62. Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport.
    J Cell Biol. 1995 Nov;131(4):875-93 PMID: 7490291
  63. Staphylococcal alpha-toxin, streptolysin-O, and Escherichia coli hemolysin: prototypes of pore-forming bacterial cytolysins.
    Arch Microbiol. 1996 Feb;165(2):73-9 PMID: 8593102
  64. Coat proteins and vesicle budding.
    Science. 1996 Mar 15;271(5255):1526-33 PMID: 8599108
  65. An endosomal beta COP is involved in the pH-dependent formation of transport vesicles destined for late endosomes.
    J Cell Biol. 1996 Apr;133(1):29-41 PMID: 8601610
  66. Protein sorting by transport vesicles.
    Science. 1996 Apr 12;272(5259):227-34 PMID: 8602507
  67. A single point mutation in epsilon-COP results in temperature-sensitive, lethal defects in membrane transport in a Chinese hamster ovary cell mutant.
    J Biol Chem. 1996 May 10;271(19):11191-6 PMID: 8626666
  68. The Hansenula polymorpha PER9 gene encodes a peroxisomal membrane protein essential for peroxisome assembly and integrity.
    J Biol Chem. 1996 Apr 12;271(15):8887-94 PMID: 8621531
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.141.2.373
Published
1998-04-20
Pages
373-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148451
Subset
IM
Databases
GENBANK
AJ224120
External Links
PubMed source DOI: 10.1083/jcb.141.2.373 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2