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PMID: 8601610 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An endosomal beta COP is involved in the pH-dependent formation of transport vesicles destined for late endosomes.

The Journal of cell biology ·Vol. 133 ·No. 1 ·1996-04-00 ·Pages 29-41

Aniento F, Gu F, Parton RG, Gruenberg J

Abstract

In this paper, we show that beta COP is present on endosomes and is required for the formation of vesicles which mediate transport from early to late endosomes. Both the association of beta COP to endosomal membranes as well as transport vesicle formation depend on the lumenal pH. We find that epsilon COP, but not gamma COP, is also associated to endosomes, and that this association is also lumenal pH dependent. Our data, thus, indicate that a subset of COPs is part of the mechanism regulating endosomal membrane transport, and that membrane association of these COPs is controlled by the acidic properties of early endosomes, presumably via a trans-membrane pH sensor.

MeSH Terms
Animals Biological Transport Cell Fractionation Cell Line Coatomer Protein Cricetinae Endosomes/chemistry,metabolism Hydrogen-Ion Concentration Intracellular Membranes/metabolism Membrane Proteins/analysis,metabolism,physiology Microtubule-Associated Proteins/analysis,metabolism,physiology Vacuoles/chemistry
Chemicals
Coatomer Protein Membrane Proteins Microtubule-Associated Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aniento F
Department of Biochemistry, Sciences II, Geneva, Switzerland.
Gu F
Parton R G
Gruenberg J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.133.1.29
Published
1996-04-00
Pages
29-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120778
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.133.1.29 PMC Full Text View journal details
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