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PMID: 9537402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for a regulatory link of nitrogen fixation and photosynthesis in Rhodobacter capsulatus via HvrA.

Journal of bacteriology ·Vol. 180 ·No. 7 ·1998-04-00 ·Pages 1965-9

Kern M, Kamp PB, Paschen A, Masepohl B, Klipp W

Abstract

A Rhodobacter capsulatus reporter strain, carrying a constitutively expressed nifA gene and a nifH-lacZ gene fusion, was used for random transposon Tn5 mutagenesis to search for genes required for the NtrC-independent ammonium repression of NifA activity. A mutation in hvrA, which is known to be involved in low-light activation of the photosynthetic apparatus, released both ammonium and oxygen control of nifH expression in this reporter strain, demonstrating a regulatory link of nitrogen fixation and photosynthesis via HvrA. In addition, a significant increase in bacteriochlorophyll alpha (BChl alpha) content was found in cells under nitrogen-fixing conditions. HvrA was not involved in this up-regulation of BChl alpha. Instead, the presence of active nitrogenase seemed to be sufficient for this process, since no increase in BChl alpha content was observed in different nif mutants.

MeSH Terms
Bacterial Proteins/genetics,physiology Genes, Bacterial Nitrogen Fixation Nitrogenase/biosynthesis,genetics Oxidoreductases Oxygen/pharmacology Photosynthesis Pigments, Biological/biosynthesis Quaternary Ammonium Compounds/pharmacology Rhodobacter capsulatus/genetics,metabolism Trans-Activators/genetics,physiology
Chemicals
Bacterial Proteins HvrA protein, Rhodobacter capsulatus Pigments, Biological Quaternary Ammonium Compounds Trans-Activators Oxidoreductases Nitrogenase nitrogenase reductase Oxygen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kern M
Ruhr-Universität Bochum, Fakultät für Biologie, Lehrstuhl für Biologie der Mikroorganismen, Germany.
Kamp P B
Paschen A
Masepohl B
Klipp W
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.7.1965-1969.1998
Published
1998-04-00
Pages
1965-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107117
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.7.1965-1969.1998 PMC Full Text View journal details
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