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PMID: 2175385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An Fnr-like protein encoded in Rhizobium leguminosarum biovar viciae shows structural and functional homology to Rhizobium meliloti FixK.

Molecular & general genetics : MGG ·Vol. 223 ·No. 1 ·1990-08-00 ·Pages 138-47

Colonna-Romano S, Arnold W, Schlüter A, Boistard P, Pühler A, Priefer UB

Abstract

A 1.9 kb DNA region of Rhizobium leguminosarum biovar viciae strain VF39 capable of promoting microaerobic and symbiotic induction of the Rhizobium meliloti fixN gene was identified by heterologous complementation. Sequence analysis of this DNA region revealed the presence of two complete open reading frames, orf240 and orf114. The deduced amino acid sequence of orf240 showed significant homology to Escherichia coli Fnr and R. meliloti FixK. The major difference between ORF240 and FixK is the presence of 21 N-terminal amino acids in ORF240 that have no counterpart in FixK. A similar protein domain is also present in E. coli Fnr and is essential for the oxygen-regulated activity of this protein. Analysis of the nucleotide sequence upstream of orf240 revealed a motif similar to the NtrA-dependent promoter consensus sequence, as well as two DNA regions resembling the Fnr consensus binding sequence. A Tn5-generated mutant in orf240 lost the ability to induce the R. meliloti fixN-lacZ fusion. Interestingly, this mutant was still capable of nitrogen fixation but showed reduced nitrogenase activity.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Cloning, Molecular Consensus Sequence DNA Transposable Elements Escherichia coli Proteins Genes, Bacterial Genetic Complementation Test Iron-Sulfur Proteins Molecular Sequence Data Mutation Nitrogen Fixation/genetics Open Reading Frames Phenotype Restriction Mapping Rhizobium/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins DNA Transposable Elements Escherichia coli Proteins FNR protein, E coli Iron-Sulfur Proteins FixK protein, Bacteria
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colonna-Romano S
University of Bielefeld, Faculty of Biology, Department of Genetics, Federal Republic of Germany.
Arnold W
Schlüter A
Boistard P
Pühler A
Priefer U B
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00315806
Published
1990-08-00
Pages
138-47
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00315806
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