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PMID: 9484465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural analysis of the nit2/nit1/nit3 gene cluster encoding nitrilases, enzymes catalyzing the terminal activation step in indole-acetic acid biosynthesis in Arabidopsis thaliana.

Plant molecular biology ·Vol. 36 ·No. 1 ·1998-01-00 ·Pages 89-99

Hillebrand H, Bartling D, Weiler EW

Abstract

A 13.8 kb DNA sequence containing the promoters and the structural genes of the Arabidopsis thaliana nit2/nit1/nit3 gene cluster has been isolated and characterized. The coding regions of nit2, nit1 and nit3 spanned 1.9, 1.8 and 2.1 kb, respectively. The architecture of the three genes is highly conserved. Each isoform consists of five exons separated by four introns. The introns are very similar with respect to size and position, but differ considerably in sequence composition. In contrast to the coding sequences the three promoters are very different in sequence, size and in their repertoire of cis elements, suggesting differential regulation of the three nitrilase isoenzymes by the developmental program of the plant and by diverse environmental factors. The nit1 promoter was subjected to analysis in planta. Translational fusions placing the nit1 full-length promoter and a series of 5'-deletion fragments in front of the uidA gene encoding beta-glucuronidase (GUS) were used for Agrobacterium tumefaciens-mediated transformation of Nicotiana tabacum. GUS expression was highest in fully expanded leaves and in the shoot apex as well as in the apices of developing lateral buds, whereas the GUS activity displayed by developing younger leaflets was restricted to the tips of the expanding leaves. Within the root tissue GUS expression was restricted to the root tips and the tips of newly forming lateral roots. Structural features of the nitrilase gene family and nitrilase gene expression patterns are discussed in context with current knowledge of auxin biosynthesis and auxin effects on different tissues.

MeSH Terms
Aminohydrolases/biosynthesis,genetics,metabolism Arabidopsis/enzymology,genetics Base Sequence Cloning, Molecular DNA, Plant/chemistry Genes, Plant Genomic Library Glucuronidase/biosynthesis Indoleacetic Acids/metabolism Isoenzymes/biosynthesis,genetics,metabolism Molecular Sequence Data Multigene Family Plant Leaves Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis Tobacco/enzymology
Chemicals
DNA, Plant Indoleacetic Acids Isoenzymes Recombinant Fusion Proteins indoleacetic acid Glucuronidase Aminohydrolases nitrilase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hillebrand H
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, Germany.
Bartling D
Weiler E W
References (36)
36 references, click to expand
  1. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089-93 PMID: 2902624
  2. The plant G box promoter sequence activates transcription in Saccharomyces cerevisiae and is bound in vitro by a yeast activity similar to GBF, the plant G box binding factor.
    EMBO J. 1990 Jun;9(6):1727-35 PMID: 2161333
  3. Functional borders, genetic fine structure, and distance requirements of cis elements mediating light responsiveness of the parsley chalcone synthase promoter.
    Proc Natl Acad Sci U S A. 1990 Jul;87(14):5387-91 PMID: 2371277
  4. Two tobacco DNA-binding proteins with homology to the nuclear factor CREB.
    Nature. 1989 Aug 31;340(6236):727-30 PMID: 2528073
  5. Structure of the gene encoding nitrilase 1 from Arabidopsis thaliana.
    Gene. 1996 May 8;170(2):197-200 PMID: 8666244
  6. Transgenic tobacco plants expressing the Arabidopsis thaliana nitrilase II enzyme.
    Plant J. 1996 May;9(5):683-91 PMID: 8653117
  7. Plant bZIP proteins gather at ACGT elements.
    FASEB J. 1994 Feb;8(2):192-200 PMID: 8119490
  8. Sequence and characterization of two auxin-regulated genes from soybean.
    J Biol Chem. 1988 Aug 5;263(22):10658-66 PMID: 2899079
  9. A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes.
    EMBO J. 1991 Jul;10(7):1793-802 PMID: 2050116
  10. A plant leucine zipper protein that recognizes an abscisic acid response element.
    Science. 1990 Oct 12;250(4978):267-71 PMID: 2145628
  11. Cloning and expression of an Arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone, indole-3-acetic acid.
    Eur J Biochem. 1992 Apr 1;205(1):417-24 PMID: 1555601
  12. Binding of a pea nuclear protein to promoters of certain photoregulated genes is modulated by phosphorylation.
    Plant Cell. 1989 Nov;1(11):1069-77 PMID: 2562560
  13. Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA.
    Proc Natl Acad Sci U S A. 1990 Jan;87(1):46-50 PMID: 2296602
  14. Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene.
    EMBO J. 1987 Sep;6(9):2543-9 PMID: 3678200
  15. A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements.
    EMBO J. 1989 Jun;8(6):1641-8 PMID: 2767049
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  18. A catalogue of splice junction and putative branch point sequences from plant introns.
    Nucleic Acids Res. 1986 Dec 22;14(24):9549-59 PMID: 3808952
  19. Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter.
    Plant Physiol. 1993 Sep;103(1):97-103 PMID: 8208860
  20. Nopaline synthase promoter is wound inducible and auxin inducible.
    Plant Cell. 1990 Mar;2(3):225-33 PMID: 2152114
  21. Regulation of gene expression during plant embryogenesis.
    Cell. 1989 Jan 27;56(2):149-60 PMID: 2643470
  22. Glucosinolates and their breakdown products in food and food plants.
    Crit Rev Food Sci Nutr. 1983;18(2):123-201 PMID: 6337782
  23. The maize regulatory locus Opaque-2 encodes a DNA-binding protein which activates the transcription of the b-32 gene.
    EMBO J. 1991 Mar;10(3):617-24 PMID: 2001677
  24. Genetic approaches to auxin action.
    Plant Cell Environ. 1994 Jun;17(6):525-40 PMID: 11541116
  25. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  26. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing.
    Cell. 1989 Aug 11;58(3):473-83 PMID: 2758463
  27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  28. Organization and expression of the genes encoding ribulose-1,5-bisphosphate carboxylase in higher plants.
    Photosynth Res. 1988 Apr;16(1-2):117-39 PMID: 24430995
  29. The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer.
    EMBO J. 1987 Nov;6(11):3203-8 PMID: 16453801
  30. Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: key enzymes in biosynthesis of the plant hormone indole-3-acetic acid.
    Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6021-5 PMID: 8016109
  31. Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6649-53 PMID: 8022831
  32. TGA1 and G-box binding factors: two distinct classes of Arabidopsis leucine zipper proteins compete for the G-box-like element TGACGTGG.
    Plant Cell. 1992 Oct;4(10):1309-19 PMID: 1446171
  33. Rethinking Auxin Biosynthesis and Metabolism.
    Plant Physiol. 1995 Feb;107(2):323-329 PMID: 12228361
  34. Regulation of beta-glucuronidase expression in transgenic tobacco plants by an A/T-rich, cis-acting sequence found upstream of a French bean beta-phaseolin gene.
    Plant Cell. 1989 Sep;1(9):839-53 PMID: 2535526
  35. Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10355-9 PMID: 8234297
  36. Light-inducible and constitutively expressed DNA-binding proteins recognizing a plant promoter element with functional relevance in light responsiveness.
    EMBO J. 1991 Jul;10(7):1777-86 PMID: 2050115
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005998918418
Published
1998-01-00
Pages
89-99
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
Y07648
External Links
PubMed source DOI: 10.1023/a:1005998918418 View journal details
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