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PMID: 8022831 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 14 ·1994-07-05 ·Pages 6649-53

Bartel B, Fink GR

Abstract

Nitrilases (nitrile aminohydrolase, EC 3.5.5.1) convert nitriles to carboxylic acids. We report the cloning, characterization, and expression patterns of four Arabidopsis thaliana nitrilase genes (NIT1-4), one of which was previously described [Bartling, D., Seedorf, M., Mithöfer, A. & Weiler, E. W. (1992) Eur. J. Biochem. 205, 417-424]. The nitrilase genes encode very similar proteins that hydrolyze indole-3-acetonitrile to the phytohormone indole-3-acetic acid in vitro, and three of the four genes are tandemly arranged on chromosome III. Northern analysis using gene-specific probes and analysis of transgenic plants containing promoter-reporter gene fusions indicate that the four genes are differentially regulated. NIT2 expression is specifically induced around lesions caused by bacterial pathogen infiltration. The sites of nitrilase expression may represent sites of auxin biosynthesis in A. thaliana.

Related Genes
GUS NIT1 NIT2 NIT3 NIT4
MeSH Terms
Amino Acid Sequence Aminohydrolases/biosynthesis,genetics Arabidopsis/enzymology,genetics,growth & development Base Sequence Chromosome Mapping Cloning, Molecular DNA Primers Escherichia coli Gene Expression Regulation, Enzymologic Genes, Plant Glucuronidase/biosynthesis Indoleacetic Acids/biosynthesis Molecular Sequence Data Multigene Family Open Reading Frames Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis Recombinant Proteins/biosynthesis Restriction Mapping Sequence Homology, Amino Acid
Chemicals
DNA Primers Indoleacetic Acids Recombinant Fusion Proteins Recombinant Proteins Glucuronidase Aminohydrolases nitrilase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bartel B
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Fink G R
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.14.6649
Published
1994-07-05
Pages
6649-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44260
Subset
IM
Databases
GENBANK
U09958, U09959, U09961
External Links
PubMed source DOI: 10.1073/pnas.91.14.6649 PMC Full Text View journal details
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