Home LiteratureArticle Details
PMID: 9440525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant.

Journal of bacteriology ·Vol. 180 ·No. 2 ·1998-01-00 ·Pages 359-65

Gustafsson C, Persson BC

Abstract

An Escherichia coli mutant lacking the modified nucleotide m1G in rRNA has previously been isolated (G. R. Björk and L. A. Isaksson, J. Mol. Biol. 51:83-100, 1970). In this study, we localize the position of the m1G to nucleotide 745 in 23S rRNA and characterize a mutant deficient in this modification. This mutant shows a 40% decreased growth rate in rich media, a drastic reduction in loosely coupled ribosomes, a 20% decreased polypeptide chain elongation rate, and increased resistance to the ribosome binding antibiotic viomycin. The rrmA gene encoding 23S rRNA m1G745 methyltransferase was mapped to bp 1904000 on the E. coli chromosome and identified to be identical to the previously sequenced gene yebH.

MeSH Terms
Chromatography, High Pressure Liquid Drug Resistance, Microbial/genetics Escherichia coli/drug effects,enzymology,genetics Genetic Complementation Test Methyltransferases/genetics Microbial Sensitivity Tests Mutation Open Reading Frames RNA, Ribosomal, 23S/genetics Viomycin/pharmacology
Chemicals
RNA, Ribosomal, 23S Methyltransferases 23S rRNA m1G745 methyltransferase Viomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gustafsson C
Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Persson B C
References (31)
31 references, click to expand
  1. A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe).
    RNA. 1995 Jun;1(4):437-48 PMID: 7493321
  2. A compilation of large subunit RNA sequences presented in a structural format.
    Nucleic Acids Res. 1988;16 Suppl:r175-269 PMID: 3368328
  3. Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA.
    J Mol Biol. 1970 Jul 14;51(1):83-100 PMID: 4921251
  4. Isolation of "relaxed" mutants of Escherichia coli.
    J Bacteriol. 1968 Feb;95(2):729-31 PMID: 4867755
  5. Molecular analysis of kanamycin and viomycin resistance in Mycobacterium smegmatis by use of the conjugation system.
    J Bacteriol. 1997 Aug;179(15):4795-801 PMID: 9244267
  6. Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli.
    Nat New Biol. 1971 Sep 1;233(35):12-4 PMID: 4329247
  7. Analysis of ribosomes from viomycin-sensitive and -resistant strains of Mycobacterium smegmatis.
    J Bacteriol. 1972 Oct;112(1):1-6 PMID: 4342812
  8. Induction kinetics of the L-arabinose operon of Escherichia coli.
    J Bacteriol. 1973 Jul;115(1):9-14 PMID: 4577756
  9. Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity.
    Biochim Biophys Acta. 1973 Jun 8;312(1):122-33 PMID: 4580201
  10. Formation in vitro of 1-methylguanine in 23-s RNA from Escherichia coli. The effects of spermidine and two proteins.
    Biochim Biophys Acta. 1973 Jun 8;312(1):134-46 PMID: 4580202
  11. The inhibition of ribosomal translocation by viomycin.
    Eur J Biochem. 1977 Dec;81(3):491-7 PMID: 202460
  12. General screening procedure for RNA modificationless mutants: isolation of Escherichia coli strains with specific defects in RNA methylation.
    J Bacteriol. 1978 Feb;133(2):499-507 PMID: 342494
  13. Streptomycin- and viomycin-induced conformational changes of ribosomes detected by iodination.
    FEBS Lett. 1978 Mar 1;87(1):21-5 PMID: 204516
  14. Resistance to viomycin conferred by RNA of either ribosomal subunit.
    Nature. 1978 Oct 5;275(5679):460-1 PMID: 211438
  15. 23S ribosomal ribonucleic acid of macrolide-producing streptomycetes contains methylated adenine.
    J Bacteriol. 1979 Mar;137(3):1464-7 PMID: 438126
  16. Methylation patterns of mycoplasma transfer and ribosomal ribonucleic acid.
    J Bacteriol. 1980 Dec;144(3):991-8 PMID: 6160144
  17. Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton.
    J Biol Chem. 1982 Jul 25;257(14):7915-7 PMID: 6806287
  18. Quantitative enzymatic hydrolysis of tRNAs: reversed-phase high-performance liquid chromatography of tRNA nucleosides.
    J Chromatogr. 1982 Jul 9;230(2):297-308 PMID: 7050138
  19. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  20. Transfer RNA shields specific nucleotides in 16S ribosomal RNA from attack by chemical probes.
    Cell. 1986 Dec 26;47(6):985-94 PMID: 2430725
  21. Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA.
    Biochimie. 1987 Aug;69(8):879-84 PMID: 3122849
  22. Interaction of proteins S16, S17 and S20 with 16 S ribosomal RNA.
    J Mol Biol. 1988 Mar 20;200(2):291-9 PMID: 3373529
  23. Non-autogenous control of ribosomal protein synthesis from the trmD operon in Escherichia coli.
    J Mol Biol. 1988 Sep 5;203(1):141-52 PMID: 2460631
  24. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
    Microbiol Rev. 1989 Mar;53(1):1-24 PMID: 2540407
  25. Dominant lethal mutations in a conserved loop in 16S rRNA.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1042-6 PMID: 2405392
  26. Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components.
    Microbiol Rev. 1990 Jun;54(2):89-100 PMID: 1694554
  27. Clustering of modified nucleotides at the functional center of bacterial ribosomal RNA.
    FASEB J. 1993 Jan;7(1):161-7 PMID: 8422963
  28. Cloning and sequences of two macrolide-resistance-encoding genes from mycinamicin-producing Micromonospora griseorubida.
    Gene. 1994 Apr 8;141(1):39-46 PMID: 8163173
  29. Widespread occurrence of three sequence motifs in diverse S-adenosylmethionine-dependent methyltransferases suggests a common structure for these enzymes.
    Arch Biochem Biophys. 1994 May 1;310(2):417-27 PMID: 8179327
  30. Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins.
    Mol Microbiol. 1994 Mar;11(5):833-9 PMID: 8022261
  31. The Ribosomal Database Project (RDP).
    Nucleic Acids Res. 1996 Jan 1;24(1):82-5 PMID: 8594608
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.2.359-365.1998
Published
1998-01-00
Pages
359-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC106891
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.2.359-365.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2