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PMID: 4342812 Published · ppublish English Journal Article

Analysis of ribosomes from viomycin-sensitive and -resistant strains of Mycobacterium smegmatis.

Journal of bacteriology ·Vol. 112 ·No. 1 ·1972-10-00 ·Pages 1-6

Yamada T, Masuda K, Shoji K, Hori M

Abstract

Viomycin-resistant strains were isolated from Mycobacterium smegmatis. Ribosomes were isolated and tested for drug resistance in subcellular systems containing poly(U) as messenger ribonucleic acid. Resistance to viomycin in these strains was due to altered ribosomes. Further analysis showed that viomycin resistance of two mutants with low level resistance (20 mug/ml) was due to altered 30S ribosomal subunits. Another mutant that was highly resistant to viomycin (1 mg/ml), however, had altered 50S ribosomal subunits.

MeSH Terms
Bacterial Proteins/biosynthesis Carbon Isotopes Cell-Free System Centrifugation, Density Gradient Drug Resistance, Microbial Escherichia coli Genetics, Microbial Magnesium Micropore Filters Mutation Mycobacterium/drug effects,metabolism Peptide Biosynthesis Phenylalanine/metabolism Poly U RNA, Transfer Ribosomes/drug effects,metabolism Viomycin/pharmacology
Chemicals
Bacterial Proteins Carbon Isotopes Poly U Phenylalanine RNA, Transfer Magnesium Viomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamada T
Masuda K
Shoji K
Hori M
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.112.1.1-6.1972
Published
1972-10-00
Pages
1-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251373
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.112.1.1-6.1972 PMC Full Text View journal details
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