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PMID: 2540407 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.

Microbiological reviews ·Vol. 53 ·No. 1 ·1989-03-00 ·Pages 1-24

Singer M, Baker TA, Schnitzler G, Deischel SM, Goel M, Dove W, Jaacks KJ, Grossman AD, Erickson JW, Gross CA

Abstract

We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most positions both Tn10 (Tetr) and TN10kan (Kanr) elements are available, so that the collection contains a linked set of alternating antibiotic resistance markers. The map position of each insertion has been aligned to the E. coli genetic map as well as to the Kohara ordered clone bank. These strains are designed to be used in a rapid two-step mapping system in E. coli. In the first step, the mutation is localized to a 5- to 15-min region of the chromosome by Hfr mapping with a set of Hfr strains containing either Tn10 or Tn10kan elements located 20 min from their respective origins of transfer. In the second step, the mutation is localized to a 1-min region by P1 transduction, with a collection of isogenic insertion strains as donors. We discuss the uses of this collection of strains to map and eventually to clone a variety of mutations in E. coli.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA Transposable Elements Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics Mutation R Factors Transduction, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Singer M
Baker T A
Schnitzler G
Deischel S M
Goel M
Dove W
Jaacks K J
Grossman A D
Erickson J W
Gross C A
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Article Info
Journal
Microbiological reviews
Abbr.
Microbiol Rev
ISSN
0146-0749
DOI
10.1128/mr.53.1.1-24.1989
Published
1989-03-00
Pages
1-24
Language
English
Region
United States
NLM ID
7806086
PMCID
PMC372715
Subset
IM
Grants
NIAID NIH HHS · 5 RO1 AI19635 · United States
External Links
PubMed source DOI: 10.1128/mr.53.1.1-24.1989 PMC Full Text
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