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PMID: 9425157 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit.

The Journal of cell biology ·Vol. 140 ·No. 1 ·1998-01-12 ·Pages 91-9

Vashishtha M, Phalen T, Marquardt MT, Ryu JS, Ng AC, Kielian M

Abstract

Membrane fusion and budding are key steps in the life cycle of all enveloped viruses. Semliki Forest virus (SFV) is an enveloped alphavirus that requires cellular membrane cholesterol for both membrane fusion and efficient exit of progeny virus from infected cells. We selected an SFV mutant, srf-3, that was strikingly independent of cholesterol for growth. This phenotype was conferred by a single amino acid change in the E1 spike protein subunit, proline 226 to serine, that increased the cholesterol independence of both srf-3 fusion and exit. The srf-3 mutant emphasizes the relationship between the role of cholesterol in membrane fusion and virus exit, and most significantly, identifies a novel spike protein region involved in the virus cholesterol requirement.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cholesterol/metabolism Cricetinae Membrane Fusion/physiology Molecular Sequence Data Point Mutation Proline Semliki forest virus/genetics,physiology Sequence Alignment Sequence Homology, Amino Acid Serine Viral Envelope Proteins/chemistry,genetics,metabolism Virus Replication
Chemicals
Viral Envelope Proteins Serine Cholesterol Proline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vashishtha M
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Phalen T
Marquardt M T
Ryu J S
Ng A C
Kielian M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.140.1.91
Published
1998-01-12
Pages
91-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132589
Subset
IM
Grants
NHLBI NIH HHS · 15T32 HL-07675 · United States
NCI NIH HHS · 2T32 CA09173-15 · United States
NCI NIH HHS · P30-CA13330 · United States
External Links
PubMed source DOI: 10.1083/jcb.140.1.91 PMC Full Text View journal details
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