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PMID: 7867069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleocapsid and glycoprotein organization in an enveloped virus.

Cell ·Vol. 80 ·No. 4 ·1995-02-24 ·Pages 621-30

Cheng RH, Kuhn RJ, Olson NH, Rossmann MG, Choi HK, Smith TJ, Baker TS

Abstract

Alphaviruses are a group of icosahedral, positive-strand RNA, enveloped viruses. The membrane bilayer, which surrounds the approximately 400 A diameter nucleocapsid, is penetrated by 80 spikes arranged in a T = 4 lattice. Each spike is a trimer of heterodimers consisting of glycoproteins E1 and E2. Cryoelectron microscopy and image reconstruction of Ross River virus showed that the T = 4 quaternary structure of the nucleocapsid consists of pentamer and hexamer clusters of the capsid protein, but not dimers, as have been observed in several crystallographic studies. The E1-E2 heterodimers form one-to-one associations with the nucleocapsid monomers across the lipid bilayer. Knowledge of the atomic structure of the capsid protein and our reconstruction allows us to identify capsid-protein residues that interact with the RNA, the glycoproteins, and adjacent capsid-proteins.

MeSH Terms
Freezing Glycoproteins/ultrastructure Image Processing, Computer-Assisted/methods Microscopy, Electron/methods Models, Structural Ross River virus/ultrastructure Viral Proteins/ultrastructure
Chemicals
Glycoproteins Viral Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cheng R H
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Kuhn R J
Olson N H
Rossmann M G
Choi H K
Smith T J
Baker T S
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(95)90516-2
Published
1995-02-24
Pages
621-30
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC4167723
Subset
IM
Grants
NIGMS NIH HHS · R37 GM033050 · United States
NIGMS NIH HHS · R01 GM033050 · United States
NIAID NIH HHS · AI11217 · United States
NIGMS NIH HHS · GM33050 · United States
NIAID NIH HHS · AI33982 · United States
NIAID NIH HHS · P01 AI035212 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(95)90516-2 PMC Full Text View journal details
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