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PMID: 8389472 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Lipid composition and fluidity of the human immunodeficiency virus envelope and host cell plasma membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 11 ·1993-06-01 ·Pages 5181-5

Aloia RC, Tian H, Jensen FC

Abstract

Previous studies have indicated that human immunodeficiency virus (HIV) is enclosed with a lipid envelope similar in composition to cell plasma membranes and to other viruses. Further, the fluidity, as measured by spin resonance spectroscopy, is low and the viral envelope is among the most highly ordered membranes analyzed. However, the relationship between viral envelope lipids and those of the host cell is not known. Here we demonstrate that the phospholipids within the envelopes of HIV-1RF and HIV-2-L are similar to each other but significantly different from their respective host cell surface membranes. Further, we demonstrate that the cholesterol-to-phospholipid molar ratio of the viral envelope is approximately 2.5 times that of the host cell surface membranes. Consistent with the elevated cholesterol-to-phospholipid molar ratio, the viral envelopes of HIV-1RF and HIV-2-L were shown to be 7.5% and 10.5% more ordered than the plasma membranes of their respective host cells. These data demonstrate that HIV-1 and HIV-2-L select specific lipid domains within the surface membrane of their host cells through which to emerge during viral maturation.

MeSH Terms
Animals Cell Line Cell Membrane/chemistry,metabolism Cell Transformation, Viral Cholesterol/analysis,metabolism Electron Spin Resonance Spectroscopy Female HIV/genetics,isolation & purification,metabolism HIV-1/genetics,isolation & purification,metabolism HIV-2/genetics,isolation & purification,metabolism Humans Lipid Metabolism Lipids/isolation & purification Male Membrane Fluidity Membrane Lipids/analysis Phospholipids/analysis,metabolism Primates Simian Immunodeficiency Virus/isolation & purification,metabolism
Chemicals
Lipids Membrane Lipids Phospholipids Cholesterol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aloia R C
Anesthesia Service, J. L. Pettis Veterans Administration Hospital, Loma Linda, CA 92357.
Tian H
Jensen F C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.11.5181
Published
1993-06-01
Pages
5181-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46679
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.11.5181 PMC Full Text View journal details
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