Home LiteratureArticle Details
PMID: 2829209 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Lipid composition and fluidity of the human immunodeficiency virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 3 ·1988-02-00 ·Pages 900-4

Aloia RC, Jensen FC, Curtain CC, Mobley PW, Gordon LM

Abstract

Lipid analyses of the human immunodeficiency virus (HIV) propagated in Hut 78 cells indicated a low total lipid/protein ratio, a high cholesterol/phospholipid molar ratio, and major phospholipids consisting of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and phosphatidylserine; comparable lipid profiles were noted for human erythrocytes and other RNA viruses. Electron spin resonance (ESR) studies of HIV labeled with 5-nitroxide stearate (N-oxy-4',4'-dimethyloxazolidine derivative of ketostearate) showed a low "fluidity" at 37 degrees C, similar to other enveloped RNA viruses and erythrocytes and probably due to the high cholesterol/phospholipid ratio. Ethanol (50%) completely disrupts the envelope, contributing to the rapid inactivation of HIV by ethanol. Contrarily, heating to 57 degrees C causes much less fluidization, and this heating may play a role in the slower viral inactivation at high temperatures. Should a critical minimum ordering in the HIV envelope be necessary for viral stability and infectivity, manipulating the lipid composition or fluidizing the HIV membrane, or both, may provide an untried therapeutic approach.

MeSH Terms
Cholesterol/analysis Chromatography, Thin Layer Electron Spin Resonance Spectroscopy Ethanol/pharmacology HIV/analysis,drug effects,ultrastructure Hot Temperature Membrane Fluidity Membrane Lipids/analysis Phospholipids/analysis Spin Labels Virulence
Chemicals
Membrane Lipids Phospholipids Spin Labels Ethanol Cholesterol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aloia R C
Anesthesiology Service, J. L. Pettis Memorial Veterans Administration Hospital, Loma Linda, CA 92357.
Jensen F C
Curtain C C
Mobley P W
Gordon L M
References (36)
36 references, click to expand
  1. The effect of the antioxidant butylated hydroxy toluene on serum lipid and glycoprotein levels in the rat.
    Aust J Exp Biol Med Sci. 1959 Jun;37:295-305 PMID: 13814589
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Precise quantitative determination of human blood lipids by thin-layer and triethylaminoethylcellulose column chromatography. I. Erythrocyte lipids.
    Anal Biochem. 1970 Dec;38(2):423-36 PMID: 5493067
  4. Perturbations of the dynamics of lipid alkyl chains in membrane systems: effect on the activity of membrane-bound enzymes.
    Biochim Biophys Acta. 1974 Mar 15;339(2):190-201 PMID: 4611490
  5. Effect of membrane protein on lipid bilayer structure: a spin-label electron spin resonance study of vesicular stomatitis virus.
    Biochemistry. 1976 Jun 1;15(11):2356-60 PMID: 179567
  6. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
    Exp Cell Res. 1977 Feb;104(2):255-62 PMID: 65285
  7. Spin-label studies on rat liver and heart plasma membranes: do probe-probe interactions interfere with the measurement of membrane properties?
    J Membr Biol. 1977 Feb 24;31(1-2):131-69 PMID: 190406
  8. Studies on spin-labelled egg lecithin dispersions.
    Biochim Biophys Acta. 1977 Apr 1;466(1):34-43 PMID: 192293
  9. Butylated hydroxytoluene protects chickens exposed to Newcastle disease virus.
    Science. 1977 Sep 23;197(4310):1291-2 PMID: 897670
  10. Lipid organization of the membrane of vesicular stomatitis virus.
    J Biol Chem. 1978 Jul 10;253(13):4544-50 PMID: 207703
  11. Inactivation of cytomegalovirus and Semliki Forest virus by butylated hydroxytoluene.
    J Infect Dis. 1978 Jul;138(1):91-4 PMID: 210237
  12. Interaction of vesicular stomatitis virus with lipid vesicles: depletion of cholesterol and effect on virion membrane fluidity and infectivity.
    J Virol. 1978 Aug;27(2):320-9 PMID: 211263
  13. Amphoptericin B has very different effects on the glucagon and fluoride-stimulated adenylate cyclase activities of rat liver plasma membranes.
    FEBS Lett. 1979 Oct 1;106(1):21-4 PMID: 499495
  14. Effects of calcium, lanthanum, and temperature on the fluidity of spin-labeled human platelets.
    J Membr Biol. 1980;52(3):201-19 PMID: 6247493
  15. Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line.
    Virology. 1981 Jul 15;112(1):355-60 PMID: 6166122
  16. The local anaesthetic and bilayer fluidising agent, benzyl alcohol decreases the thermostability of the integral membrane protein adenylate cyclase.
    FEBS Lett. 1982 Apr 5;140(1):85-8 PMID: 7084459
  17. The antiviral effectiveness of butylated hydroxytoluene on herpes cutaneous infections in hairless mice.
    Proc Soc Exp Biol Med. 1982 Jun;170(2):237-44 PMID: 6178117
  18. A comparison of the mobilities and thermal transitions of retrovirus lipid envelopes and host cell plasma membranes by electron spin resonance spectroscopy.
    Biochim Biophys Acta. 1982 Jul 28;689(2):393-402 PMID: 6288099
  19. Elevated membrane cholesterol concentrations inhibit glucagon-stimulated adenylate cyclase.
    Biochem J. 1983 Feb 15;210(2):437-49 PMID: 6305341
  20. Thermotropic lipid phase separations in human erythrocyte ghosts and cholesterol-enriched rat liver plasma membranes.
    J Membr Biol. 1984;79(1):75-86 PMID: 6330365
  21. A special lipid mixture for membrane fluidization.
    Biochim Biophys Acta. 1985 Jan 10;812(1):133-8 PMID: 3838139
  22. Inactivation of lymphadenopathy-associated virus by heat, gamma rays, and ultraviolet light.
    Lancet. 1985 Jan 26;1(8422):188-9 PMID: 2857267
  23. Effect of chronic alcohol consumption on rat brain microsome lipid composition, membrane fluidity and Na+-K+-ATPase activity.
    Life Sci. 1985 Mar 11;36(10):1003-17 PMID: 2983158
  24. A sensitive enzymatic assay for determination of cholesterol in lipid extracts.
    Anal Biochem. 1984 Nov 1;142(2):347-50 PMID: 6528973
  25. Spin probe clustering in human erythrocyte ghosts.
    J Membr Biol. 1985;84(1):81-95 PMID: 2987502
  26. Thermal inactivation of the acquired immunodeficiency syndrome virus, human T lymphotropic virus-III/lymphadenopathy-associated virus, with special reference to antihemophilic factor.
    J Clin Invest. 1985 Aug;76(2):875-7 PMID: 2993366
  27. Effects of a novel compound (AL 721) on HTLV-III infectivity in vitro.
    N Engl J Med. 1985 Nov 14;313(20):1289-90 PMID: 2414659
  28. Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus.
    Science. 1986 Feb 7;231(4738):589-94 PMID: 3003905
  29. Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments.
    JAMA. 1986 Apr 11;255(14):1887-91 PMID: 2419594
  30. HTLV-III and the etiology of AIDS.
    Prog Allergy. 1986;37:1-45 PMID: 3008160
  31. Lymphadenopathy associated virus: its role in the pathogenesis of AIDS and related diseases.
    Prog Allergy. 1986;37:46-64 PMID: 3515354
  32. Isolation of a new human retrovirus from West African patients with AIDS.
    Science. 1986 Jul 18;233(4761):343-6 PMID: 2425430
  33. Envelope perturbation and AIDS.
    Lancet. 1986 Nov 15;2(8516):1159 PMID: 2877302
  34. Anti-viral activity of amphotericin B methyl ester: inhibition of HTLV-III replication in cell culture.
    Biochem Pharmacol. 1986 Nov 15;35(22):4110-3 PMID: 3640625
  35. Electron spin resonance spectroscopy in the study of lymphoid cell receptors.
    Methods Enzymol. 1987;150:418-46 PMID: 2828826
  36. The lipids of paramyxoviruses: a comparative study of Sendai and Newcastle disease viruses.
    Virology. 1968 Oct;36(2):286-92 PMID: 4300882
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.3.900
Published
1988-02-00
Pages
900-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279664
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.3.900 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2