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PMID: 9409833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila male-specific lethal-2 protein: structure/function analysis and dependence on MSL-1 for chromosome association.

Genetics ·Vol. 147 ·No. 4 ·1997-12-00 ·Pages 1743-53

Lyman LM, Copps K, Rastelli L, Kelley RL, Kuroda MI

Abstract

MSL-2 is required for the male-specific assembly of a dosage compensation regulatory complex on the X chromosome of Drosophila melanogaster. We found that MSL-2 binds in a reproducible, partial pattern to the male X chromosome in the absence of MLE or MSL-3, or when ectopically expressed at a low level in females. Moreover, the pattern of MSL-2 binding corresponds precisely in each case to that of MSL-1, suggesting that the two proteins function together to associate with the X. Consistent with this hypothesis, we isolated EMS-induced loss of function msl-1 and msl-2 alleles in a screen for suppressors of the toxic effects of MSL-2 expression in females. We also used site-directed mutagenesis to determine the importance of the MSL-2 RING finger domain and second cysteine-rich motif. The mutations, including those in conserved zinc coordinating cysteines, confirm that the RING finger is essential for MSL-2 function, while suggesting a less stringent requirement for an intact second motif.

MeSH Terms
Amino Acid Sequence Animals Chromosome Mapping Cysteine/metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Drosophila Proteins Drosophila melanogaster/genetics,metabolism Female Male Molecular Sequence Data Mutagenesis Nuclear Proteins/chemistry,genetics,metabolism Structure-Activity Relationship Transcription Factors/chemistry,genetics,metabolism Zinc Fingers
Chemicals
DNA-Binding Proteins Drosophila Proteins Nuclear Proteins Transcription Factors msl-1 protein, Drosophila msl-2 protein, Drosophila Cysteine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lyman L M
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Copps K
Rastelli L
Kelley R L
Kuroda M I
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/147.4.1743
Published
1997-12-00
Pages
1743-53
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208343
Subset
IM
Grants
NIGMS NIH HHS · GM-45744 · United States
External Links
PubMed source DOI: 10.1093/genetics/147.4.1743 PMC Full Text View journal details
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