Home LiteratureArticle Details
PMID: 1313147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extra sequences found at P element excision sites in Drosophila melanogaster.

Molecular & general genetics : MGG ·Vol. 232 ·No. 1 ·1992-03-00 ·Pages 17-23

Takasu-Ishikawa E, Yoshihara M, Hotta Y

Abstract

We have previously established a transgenic Drosophila line with a highly transposable P element insertion. Using this strain we analyzed transposition and excision of the P element at the molecular level. We examined sequences flanking the new insertion sites and those of the remnants after excision. Our results on mobilization of the P element demonstrate that target-site duplication at the original insertion site does not play a role in forward excision and transposition. After P element excision an 8 bp target-site duplication and part of the 31 bp terminal inverted repeat (5-18 bp) remained in all the strains examined. Moreover, in 11 out of 28 strains, extra sequences were found between the two remaining inverted repeats. The double-strand gap repair model does not explain the origin of these extra sequences. The mechanism creating them may be similar to the hairpin model proposed for the transposon Tam in Antirrhinum majus.

MeSH Terms
Animals Base Sequence DNA Repair/genetics DNA Transposable Elements/genetics Drosophila melanogaster/genetics Molecular Sequence Data Nucleic Acid Conformation Repetitive Sequences, Nucleic Acid/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takasu-Ishikawa E
Department of Physics, Faculty of Science, University of Tokyo, Japan.
Yoshihara M
Hotta Y
References (23)
23 references, click to expand
  1. cis-acting DNA sequence requirements for P-element transposition.
    Genes Dev. 1989 May;3(5):729-38 PMID: 2545527
  2. P transposons controlled by the heat shock promoter.
    Mol Cell Biol. 1986 May;6(5):1640-9 PMID: 3023899
  3. P element excision in Drosophila melanogaster and related drosophilids.
    Mol Gen Genet. 1991 Mar;225(3):387-94 PMID: 1850084
  4. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing.
    Cell. 1986 Jan 17;44(1):7-19 PMID: 3000622
  5. Insertion of an LrDNA gene fragment and of filler DNA at a mitochondrial exon-intron junction in Podospora.
    Nucleic Acids Res. 1990 Feb 25;18(4):779-83 PMID: 2156230
  6. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 PMID: 3174659
  7. Identification and immunochemical analysis of biologically active Drosophila P element transposase.
    Cell. 1986 Jan 17;44(1):21-32 PMID: 2416475
  8. Comparison of filler DNA at immune, nonimmune, and oncogenic rearrangements suggests multiple mechanisms of formation.
    Mol Cell Biol. 1989 Jul;9(7):3049-57 PMID: 2550794
  9. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  10. Transposition in plants: a molecular model.
    EMBO J. 1985 Mar;4(3):585-90 PMID: 15926218
  11. A transposable P vector that confers selectable G418 resistance to Drosophila larvae.
    EMBO J. 1985 Jan;4(1):167-71 PMID: 16453599
  12. Dysgenesis-induced instability of rosy locus transformation in Drosophila melanogaster: analysis of excision events and the selective recovery of control element deletions.
    Genetics. 1985 Jan;109(1):95-117 PMID: 2981758
  13. The P family of transposable elements in Drosophila.
    Annu Rev Genet. 1983;17:315-44 PMID: 6320712
  14. Independence of excision frequency and transposition frequency of P element in Drosophila melanogaster.
    Jpn J Genet. 1991 Oct;66(5):535-50 PMID: 1663777
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Molecular mechanisms regulating Drosophila P element transposition.
    Annu Rev Genet. 1990;24:543-78 PMID: 1965103
  17. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  18. Frequent Imprecise Excision among Reversions of a P Element-Caused Lethal Mutation in Drosophila.
    Genetics. 1984 Jun;107(2):279-94 PMID: 17246216
  19. High-frequency P element loss in Drosophila is homolog dependent.
    Cell. 1990 Aug 10;62(3):515-25 PMID: 2165865
  20. A stable genomic source of P element transposase in Drosophila melanogaster.
    Genetics. 1988 Mar;118(3):461-70 PMID: 2835286
  21. Cytotype control of Drosophila P element transposition: the 66 kd protein is a repressor of transposase activity.
    Cell. 1990 Jul 27;62(2):269-84 PMID: 2164887
  22. Filler DNA is associated with spontaneous deletions in maize.
    Proc Natl Acad Sci U S A. 1990 Nov;87(22):8731-5 PMID: 2247441
  23. A semi-dominant allele, niv-525, acts in trans to inhibit expression of its wild-type homologue in Antirrhinum majus.
    EMBO J. 1988 Apr;7(4):877-83 PMID: 3402437
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00299132
Published
1992-03-00
Pages
17-23
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00299132
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2