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PMID: 9382901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Caffeine-induced release of intracellular Ca2+ from Chinese hamster ovary cells expressing skeletal muscle ryanodine receptor. Effects on full-length and carboxyl-terminal portion of Ca2+ release channels.

The Journal of general physiology ·Vol. 110 ·No. 6 ·1997-12-00 ·Pages 749-62

Bhat MB, Zhao J, Zang W, Balke CW, Takeshima H, Wier WG, Ma J

Abstract

The ryanodine receptor (RyR)/Ca2+ release channel is an essential component of excitation-contraction coupling in striated muscle cells. To study the function and regulation of the Ca2+ release channel, we tested the effect of caffeine on the full-length and carboxyl-terminal portion of skeletal muscle RyR expressed in a Chinese hamster ovary (CHO) cell line. Caffeine induced openings of the full length RyR channels in a concentration-dependent manner, but it had no effect on the carboxyl-terminal RyR channels. CHO cells expressing the carboxyl-terminal RyR proteins displayed spontaneous changes of intracellular [Ca2+]. Unlike the native RyR channels in muscle cells, which display localized Ca2+ release events (i.e., "Ca2+ sparks" in cardiac muscle and "local release events" in skeletal muscle), CHO cells expressing the full length RyR proteins did not exhibit detectable spontaneous or caffeine-induced local Ca2+ release events. Our data suggest that the binding site for caffeine is likely to reside within the amino-terminal portion of RyR, and the localized Ca2+ release events observed in muscle cells may involve gating of a group of Ca2+ release channels and/or interaction of RyR with muscle-specific proteins.

MeSH Terms
Animals CHO Cells/chemistry,physiology Caffeine/pharmacology Calcium/metabolism Central Nervous System Stimulants/pharmacology Cricetinae Dose-Response Relationship, Drug Electrophysiology Fura-2 Green Fluorescent Proteins Heart Ventricles/cytology Image Processing, Computer-Assisted Intracellular Membranes/chemistry Ion Channel Gating/drug effects,physiology Luminescent Proteins Muscle Fibers, Skeletal/chemistry,physiology Protein Structure, Tertiary Rabbits Rats Ryanodine Receptor Calcium Release Channel/chemistry,genetics,metabolism
Chemicals
Central Nervous System Stimulants Luminescent Proteins Ryanodine Receptor Calcium Release Channel Green Fluorescent Proteins Caffeine Calcium Fura-2
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bhat M B
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Zhao J
Zang W
Balke C W
Takeshima H
Wier W G
Ma J
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.110.6.749
Published
1997-12-00
Pages
749-62
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2229395
Subset
IM
Grants
NIA NIH HHS · AG-15556 · United States
NHLBI NIH HHS · HL-55280 · United States
External Links
PubMed source DOI: 10.1085/jgp.110.6.749 PMC Full Text View journal details
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