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PMID: 2448641 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification and reconstitution of the calcium release channel from skeletal muscle.

Nature ·Vol. 331 ·No. 6154 ·1988-01-28 ·Pages 315-9

Lai FA, Erickson HP, Rousseau E, Liu QY, Meissner G

Abstract

The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca2+-release channels.

MeSH Terms

Animals Calcium/metabolism Ion Channels/analysis,metabolism,ultrastructure Liposomes Membrane Potentials Rabbits Receptors, Cholinergic/isolation & purification,metabolism Ryanodine Receptor Calcium Release Channel Sarcoplasmic Reticulum/analysis,ultrastructure

Chemicals

Ion Channels Liposomes Receptors, Cholinergic Ryanodine Receptor Calcium Release Channel Calcium

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lai F A

Department of Biochemistry, University of North Carolina School of Medicine, Chapel Hill 27599.

Erickson H P

Rousseau E

Liu Q Y

Meissner G

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/331315a0

Published

1988-01-28

Pages

315-9

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/331315a0 View journal details

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Purification and reconstitution of the calcium release channel from skeletal muscle.

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