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PMID: 9334320 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

AU-rich elements target small nuclear RNAs as well as mRNAs for rapid degradation.

Genes & development ·Vol. 11 ·No. 19 ·1997-10-01 ·Pages 2557-68

Fan XC, Myer VE, Steitz JA

Abstract

AU-rich elements (AREs, usually containing repeated copies of AUUUA), when present in the 3'-untranslated regions (UTRs) of many mammalian mRNAs, confer instability on their host RNA molecules. The viral small nuclear RNA (snRNA) Herpesvirus saimiri U RNA 1 (HSUR 1) also contains an AUUUA-rich sequence. Here, we report that this ARE induces rapid degradation of HSUR 1 itself and of other snRNAs including HSUR 2 and cellular U1. Mutational analyses of the viral ARE establish that sequence requirements for mRNA and snRNA decay are the same, suggesting a similar mechanism. Moreover, the in vivo degradation activity of mutant AREs correlates with their in vitro binding to the HuR protein, implicated previously as a component of the mRNA degradation machinery. Our results suggest that ARE-mediated instability can be uncoupled from both ongoing translation and deadenylation of the target RNA.

MeSH Terms
Antigens, Surface Base Sequence ELAV Proteins ELAV-Like Protein 1 Gene Expression Regulation/genetics Genes, Reporter Globins/genetics Herpesvirus 2, Saimiriine/chemistry,genetics Molecular Sequence Data Mutation RNA, Messenger/genetics,metabolism RNA, Small Nuclear/genetics,metabolism RNA, Viral/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Repetitive Sequences, Nucleic Acid Ribonucleases/metabolism Transcription, Genetic Transfection
Chemicals
Antigens, Surface ELAV Proteins ELAV-Like Protein 1 ELAVL1 protein, human RNA, Messenger RNA, Small Nuclear RNA, Viral RNA-Binding Proteins Globins Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fan X C
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Myer V E
Steitz J A
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.11.19.2557
Published
1997-10-01
Pages
2557-68
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316563
Subset
IM
Grants
NCI NIH HHS · CA 16038 · United States
External Links
PubMed source DOI: 10.1101/gad.11.19.2557 PMC Full Text View journal details
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