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PMID: 1311093 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Viral small nuclear ribonucleoproteins bind a protein implicated in messenger RNA destabilization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 4 ·1992-02-15 ·Pages 1296-300

Myer VE, Lee SI, Steitz JA

Abstract

Herpesvirus saimiri (HVS) is one of several primate viruses that carry genes for small RNAs. The five H. saimiri-encoded U RNAs (HSURs) are the most abundant viral transcripts expressed in transformed marmoset T lymphocytes. They assemble with host proteins common to spliceosomal small nuclear ribonucleoproteins (snRNPs). HSURs 1, 2, and 5 exhibit sequences at their 5' ends identical to the AUUUA motif, which targets a number of protooncogene, cytokine, and lymphokine mRNAs for rapid degradation. We show that a 32-kDa protein previously demonstrated to bind to the 3' untranslated region of several unstable messages can be UV crosslinked specifically to HSUR 1, 2, and 5 transcripts in vitro, as well as to endogenous HSUR snRNPs. Our results suggest an unusual role for these viral snRNPs: HSURs may function to attenuate the rapid degradation of certain cellular mRNAs, thereby facilitating viral transformation of host T lymphocytes.

MeSH Terms
Base Sequence Binding Sites Cloning, Molecular Herpesvirus 2, Saimiriine/genetics Molecular Sequence Data Nuclear Proteins/metabolism Protein Binding RNA, Messenger/metabolism RNA, Viral/metabolism Ribonucleoproteins/metabolism,radiation effects Ribonucleoproteins, Small Nuclear Ultraviolet Rays
Chemicals
Nuclear Proteins RNA, Messenger RNA, Viral Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Myer V E
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, Congress Avenue, New Haven, CT 06536-0182.
Lee S I
Steitz J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.4.1296
Published
1992-02-15
Pages
1296-300
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48436
Subset
IM
Grants
NCI NIH HHS · CA 16038 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.4.1296 PMC Full Text View journal details
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