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PMID: 9324265 Published · ppublish English Journal Article

A 3-(3-hydroxyphenyl)propionic acid catabolic pathway in Rhodococcus globerulus PWD1: cloning and characterization of the hpp operon.

Journal of bacteriology ·Vol. 179 ·No. 19 ·1997-10-00 ·Pages 6145-53

Barnes MR, Duetz WA, Williams PA

Abstract

Rhodococcus globerulus PWD1, a soil isolate from a polluted site in The Netherlands, is able to degrade a broad range of aromatic compounds. A novel gene cluster which appears to encode a pathway for the degradation of phenolic acids such as 3-(3-hydroxyphenyl)propionate (3HPP) has been cloned from the chromosome of this organism. Sequence analysis of a 7-kb region identified five open reading frames (ORFs). Analysis of mRNA showed that the genes were expressed during growth on 3HPP and 3-hydroxyphenylacetate (3HPA) but not during growth on m-cresol or succinate. The first ORF, hppA, which appears to be separately transcribed, had considerable amino acid identity with a number of hydroxylases. Transcriptional analysis indicates that the next four ORFs, hppCBKR, which are tightly clustered, constitute a single operon. These genes appear to encode a hydroxymuconic semialdehyde hydrolase (HppC), an extradiol dioxygenase (HppB), a membrane transport protein (HppK), and a member of the IclR family of regulatory proteins (HppR). The activities of HppB and HppC have been confirmed by enzyme assay of Escherichia coli hosts. The substrate specificity of HppB expressed from the cloned gene matches that of the meta-cleavage dioxygenase expressed from wild-type Rhodococcus grown on both 3HPP and 3HPA and is considerably more active against acid than against neutral catechols. The deduced amino acid sequences of the gene products have a recognizable homology with a broad range of enzymes and proteins involved in biodegradation and appear most similar to the mhp operon from E. coli K-12, which also encodes the degradation of 3HPP.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Cloning, Molecular Coumaric Acids/metabolism Dioxygenases Evolution, Molecular Gene Expression Regulation, Bacterial Genes, Bacterial Hydrolases/genetics,metabolism Membrane Transport Proteins/genetics,metabolism Mixed Function Oxygenases/genetics,metabolism Molecular Sequence Data Open Reading Frames Operon Organic Anion Transporters Oxygenases/genetics,metabolism Phenylacetates/metabolism Phylogeny Polymerase Chain Reaction Proteins Rhodococcus/genetics,growth & development,metabolism Sequence Alignment Sequence Homology, Nucleic Acid Substrate Specificity
Chemicals
3-hydroxyphenylacetate Bacterial Proteins Coumaric Acids HppK protein, Rhodococcus globerulus Membrane Transport Proteins Organic Anion Transporters Phenylacetates Proteins dihydro-3-coumaric acid Mixed Function Oxygenases Oxygenases Dioxygenases 3-carboxyethylcatechol 2,3-dioxygenase 3-(3-hydroxyphenyl)propionate hydroxylase Hydrolases 2-hydroxymuconate-semialdehyde hydrolase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barnes M R
School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom.
Duetz W A
Williams P A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.19.6145-6153.1997
Published
1997-10-00
Pages
6145-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179521
Subset
IM
Databases
GENBANK
U89712
External Links
PubMed source DOI: 10.1128/jb.179.19.6145-6153.1997 PMC Full Text View journal details
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