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PMID: 2185227 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the glyoxylate bypass operon: cloning and characterization of iclR.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2642-9

Sunnarborg A, Klumpp D, Chung T, LaPorte DC

Abstract

In Escherichia coli, expression of the glyoxylate bypass operon appears to be controlled, in part, by the product of iclR+. Mutations in iclR have been found to yield constitutive expression of this operon, suggesting that iclR+ encodes a repressor protein. We have cloned iclR+ by taking advantage of its tight genetic linkage with the glyoxylate bypass operon. The clone complemented a mutant allele of iclR in trans, restoring an inducible phenotype for this operon. Deletion analysis identified a region of ca. 900 base pairs that was necessary and sufficient for complementation. The nucleotide sequence of the insert was then determined. Translation of this sequence revealed an open reading frame capable of encoding a protein with Mr 29,741 preceded by a potential Shine-Dalgarno ribosome-binding site. The deduced amino acid sequence includes a region at the amino terminus that may form a helix-turn-helix motif, a structure found in many DNA-binding domains.

MeSH Terms
Amino Acid Sequence Base Sequence Citric Acid Cycle Cloning, Molecular Codon/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genotype Glyoxylates/metabolism Isocitrate Lyase/genetics Malate Synthase/genetics Molecular Sequence Data Operon Oxo-Acid-Lyases/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Codon Glyoxylates Malate Synthase Oxo-Acid-Lyases Isocitrate Lyase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sunnarborg A
Department of Biochemistry, University of Minnesota, Minneapolis 55455.
Klumpp D
Chung T
LaPorte D C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2642-2649.1990
Published
1990-05-00
Pages
2642-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208908
Subset
IM
Grants
NIDDK NIH HHS · DK01722 · United States
NIDDK NIH HHS · DK40486 · United States
Databases
GENBANK
M31761
External Links
PubMed source DOI: 10.1128/jb.172.5.2642-2649.1990 PMC Full Text View journal details
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