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PMID: 3881764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mutational studies with the trp repressor of Escherichia coli support the helix-turn-helix model of repressor recognition of operator DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 2 ·1985-01-00 ·Pages 483-7

Kelley RL, Yanofsky C

Abstract

Several classes of trp repressor mutants were selected and analyzed in vivo. Mutants that produced repressors with either enhanced or reduced activity were obtained. One class of mutants produced inactive or slightly active repressors that were trans-dominant to the wild-type repressor. The amino acid substitutions in many of these repressors were clustered in a segment of the polypeptide that is homologous to the DNA recognition domain of the lambda cro repressor. A second functionally important region of the trp repressor was identified; this region could participate in L-tryptophan binding. Observations with trpR nonsense mutants suggest that the first 67 residues of the repressor polypeptide are sufficient for subunit association.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence Binding Sites DNA/metabolism Escherichia coli/genetics Mutation Nucleic Acid Conformation Operon Protein Conformation Repressor Proteins/genetics Transcription Factors/genetics
Chemicals
Bacterial Proteins Repressor Proteins TRPR protein, E coli Transcription Factors DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelley R L
Yanofsky C
References (45)
45 references, click to expand
  1. Translational restarts: AUG reinitiation of a lac repressor fragment.
    Proc Natl Acad Sci U S A. 1972 Apr;69(4):897-901 PMID: 4554534
  2. Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1428-32 PMID: 6951187
  3. How lac repressor binds to DNA.
    Nature. 1972 Jun 9;237(5354):322-7 PMID: 4557395
  4. Cyclic AMP receptor protein: role in transcription activation.
    Science. 1984 May 25;224(4651):831-8 PMID: 6372090
  5. Many gene-regulatory proteins appear to have a similar alpha-helical fold that binds DNA and evolved from a common precursor.
    J Mol Evol. 1983;19(2):109-14 PMID: 6571216
  6. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  7. Homology between CAP and Fnr, a regulator of anaerobic respiration in Escherichia coli.
    J Mol Biol. 1983 May 15;166(2):241-7 PMID: 6343617
  8. Tight-binding repressors of the lactose operon.
    J Mol Biol. 1976 Aug 5;105(2):293-319 PMID: 787534
  9. Rifampin resistance mutations that alter the efficiency of transcription termination at the tryptophan operon attenuator.
    J Bacteriol. 1981 Mar;145(3):1334-41 PMID: 7009579
  10. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
    Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
  11. Genetic studies of the lac repressor. X. Analysis of missense mutations in the lacI gene.
    J Mol Biol. 1979 Jun 25;131(2):223-48 PMID: 114666
  12. Functional inferences from crystals of Escherichia coli trp repressor.
    J Biol Chem. 1983 Oct 25;258(20):12641-3 PMID: 6355089
  13. The operator-binding domain of lambda repressor: structure and DNA recognition.
    Nature. 1982 Jul 29;298(5873):443-7 PMID: 7088190
  14. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  15. Two helix DNA binding motif of CAP found in lac repressor and gal repressor.
    Nucleic Acids Res. 1982 Aug 25;10(16):5085-102 PMID: 6897114
  16. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe.
    J Bacteriol. 1984 Jun;158(3):1018-24 PMID: 6233264
  17. Characteristics of tight binding repressors of the lac operon.
    J Mol Biol. 1981 Mar 25;147(1):1-10 PMID: 6790713
  18. Tryptophan biosynthesis in Escherichia coli. Genetic determination of the proteins involved.
    JAMA. 1971 Nov 15;218(7):1026-35 PMID: 4940311
  19. Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3097-100 PMID: 6212926
  20. Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.
    Nature. 1981 Apr 30;290(5809):744-9 PMID: 6261152
  21. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  22. Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21 PMID: 7012834
  23. Structure of the cro repressor from bacteriophage lambda and its interaction with DNA.
    Nature. 1981 Apr 30;290(5809):754-8 PMID: 6452580
  24. Proposed alpha-helical super-secondary structure associated with protein-dna recognition.
    J Mol Biol. 1982 Aug 25;159(4):745-51 PMID: 6897265
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Purification and characterization of trp aporepressor.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):668-72 PMID: 6338493
  27. Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant.
    Nucleic Acids Res. 1984 Jun 25;12(12):4849-63 PMID: 6330687
  28. An amino-terminal fragment of lac repressor binds specifically to lac operator.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851-4 PMID: 16592594
  29. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  30. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  31. Genetic studies of the lac repressor. IX. Generation of altered proteins by the suppression of nonsence mutations.
    J Mol Biol. 1979 Jun 25;131(2):191-222 PMID: 385890
  32. The lambda repressor contains two domains.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1608-12 PMID: 287002
  33. Mutations defining the operator-binding sites of bacteriophage lambda repressor.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:441-9 PMID: 6222865
  34. Amino acid replacements associated with reversion and recombination within the A gene.
    Proc Natl Acad Sci U S A. 1962 Sep 15;48:1497-504 PMID: 13906503
  35. Mutations in lambda repressor's amino-terminal domain: implications for protein stability and DNA binding.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2676-80 PMID: 6221342
  36. lac Repressor-operator interaction. Analysis of the X86 repressor mutant.
    J Mol Biol. 1976 Sep 25;106(3):857-69 PMID: 789900
  37. Primary structure of the immI immunity region of bacteriophage P22.
    J Mol Biol. 1983 Aug 25;168(4):699-713 PMID: 6350606
  38. Homology among DNA-binding proteins suggests use of a conserved super-secondary structure.
    Nature. 1982 Jul 29;298(5873):447-51 PMID: 6896364
  39. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  40. Changing the DNA-binding specificity of a repressor.
    Cell. 1983 Dec;35(3 Pt 2):777-83 PMID: 6652685
  41. The molecular basis of DNA-protein recognition inferred from the structure of cro repressor.
    Nature. 1982 Aug 19;298(5876):718-23 PMID: 6213863
  42. Trp aporepressor production is controlled by autogenous regulation and inefficient translation.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3120-4 PMID: 7048301
  43. Cro repressor protein and its interaction with DNA.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:427-33 PMID: 6305561
  44. Regulation of aromatic amino acid biosynthesis Escherichia coli K12.
    Genetics. 1968 Sep;60(1):31-48 PMID: 4884590
  45. Sequence analysis of operator constitutive mutants of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1978 May 15;121(2):179-92 PMID: 351194
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.2.483
Published
1985-01-00
Pages
483-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397063
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.2.483 PMC Full Text View journal details
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