Home LiteratureArticle Details
PMID: 927438 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mapping of restriction sites in the attachment site region of bacteriophage lambda.

Molecular & general genetics : MGG ·Vol. 154 ·No. 3 ·1977-09-09 ·Pages 231-48

Kamp D, Kahmann R, Zipser D, Roberts RJ

Abstract

A find structure map of the EcoRI fragment containing the lambda attachment-site region has been constructed. 38 different restriction endonucleases have been employed and 170 sites located in this fragment. In addition, sites in adjacent regions have been determined for several enzymes. Complete cleavage maps of the entire lambda genome have been obtained for endonucleases BglII, BluI, KpnI, SacI, SacII, SalI and XbaI. The strategy employed for mapping included comparison of deletion and substitution mutants, analysis of mixed digests, and detailed analysis of subfragments.

MeSH Terms
Chromosome Mapping Chromosomes Coliphages/genetics DNA Restriction Enzymes/genetics DNA, Viral/genetics Electrophoresis, Agar Gel Genes Mutation
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kamp D
Kahmann R
Zipser D
Roberts R J
References (42)
42 references, click to expand
  1. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  2. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
    J Mol Biol. 1975 Nov 5;98(3):551-64 PMID: 1104875
  3. Long range periodicities in mouse satellite DNA.
    J Mol Biol. 1975 May 5;94(1):51-69 PMID: 1142436
  4. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  5. Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.
    J Bacteriol. 1976 Apr;126(1):487-500 PMID: 770435
  6. Agarose slab-gel electrophoresis equipment.
    Anal Biochem. 1975 Sep;68(1):36-46 PMID: 1190452
  7. Isolation of open-circular DNA molecules by retention in agar gels.
    J Mol Biol. 1970 Sep 14;52(2):395-7 PMID: 5485916
  8. Defective bacteriophage lambda chromosome, potential vector for DNA fragments obtained after cleavage by Bacillus amyloliquefaciens endonuclease (BamI).
    FEBS Lett. 1975 Aug 1;56(1):7-11 PMID: 1157934
  9. Electron microscopy of polar insertions in the lac operon of Escherichia coli.
    Mol Gen Genet. 1972;119(3):207-22 PMID: 4567155
  10. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.
    Nucleic Acids Res. 1976 Feb;3(2):343-53 PMID: 768923
  11. The sensitivity of bacteriophage lambda DNA to restriction endonuclease RII.
    J Mol Biol. 1975 Nov 5;98(3):645-7 PMID: 1104876
  12. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.
    Nature. 1973 Jan 12;241(5385):120-3 PMID: 4572006
  13. Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.
    Biochem J. 1976 Nov;159(2):317-22 PMID: 11780
  14. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
    J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
  15. The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificities.
    Biochim Biophys Acta. 1976 Aug 18;442(2):184-96 PMID: 182257
  16. Mapping and characterization of promoters in bacteriophages fd, f1 and m13.
    J Mol Biol. 1975 Feb 25;92(2):261-77 PMID: 1095754
  17. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.
    J Mol Biol. 1973 Aug 5;78(2):363-76 PMID: 4355833
  18. Physical map and strand polarity of specific fragments of adenovirus-associated virus DNA produced by endonuclease R-EcoRI.
    J Virol. 1975 Sep;16(3):559-68 PMID: 1159894
  19. Restriction endonucleases.
    CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 PMID: 795607
  20. DNA restriction and modification mechanisms in bacteria.
    Annu Rev Microbiol. 1971;25:153-76 PMID: 4949033
  21. Fragments produced by cleavage of lambda deoxyribonucleic acid with the Hemophilus parainfluenzae restriction enzyme Hpa II.
    Biochemistry. 1973 Sep 25;12(20):3972-7 PMID: 4745657
  22. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  23. Recent advances in DNA sequence analysis.
    CRC Crit Rev Biochem. 1975 Feb;2(4):455-512 PMID: 164328
  24. Two restriction endonucleases from Bacillus globiggi.
    Nucleic Acids Res. 1976 Jul;3(7):1747-60 PMID: 967673
  25. A specific endonuclease from Arthrobacter luteus.
    J Mol Biol. 1976 Mar 25;102(1):157-65 PMID: 1271462
  26. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
    J Mol Biol. 1975 Sep 5;97(1):123-5 PMID: 1177312
  27. Lambda site-specific recombination: local transcription and an inhibitor specified by the b2 region.
    Mol Gen Genet. 1974 Jun 27;130(4):333-44 PMID: 4852640
  28. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.
    J Mol Biol. 1971 Mar 14;56(2):403-23 PMID: 5551396
  29. Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens.
    J Virol. 1976 Sep;19(3):1006-11 PMID: 787556
  30. Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
    J Mol Biol. 1975 Mar 15;92(4):529-40 PMID: 1097703
  31. A second specific endonuclease from Haemophilus aegyptius.
    J Mol Biol. 1975 Jan 5;91(1):121-3 PMID: 171410
  32. Transfection of Escherichia coli by Mu DNA.
    Mol Gen Genet. 1976 Dec 22;149(3):323-8 PMID: 799251
  33. Deletion mutants of bacteriophage lambda. II. Genetic properties of att-defective mutants.
    J Mol Biol. 1971 Mar 14;56(2):385-401 PMID: 5551395
  34. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.
    J Virol. 1972 Jul;10(1):42-50 PMID: 4537735
  35. Location in bacteriophage lamdba DNA of cleavage sites of the site-specific endonuclease from Bacillus amyloliquefaciens H.
    J Virol. 1976 May;18(2):659-63 PMID: 1083914
  36. A specific endonuclease from Haemophilus haemolyticus.
    J Mol Biol. 1976 May 5;103(1):199-208 PMID: 1085372
  37. DNA sequencing techniques.
    Annu Rev Biochem. 1974;43(0):923-65 PMID: 4604359
  38. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
    J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
  39. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  40. Int-constitutive mutants of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):237-41 PMID: 4521054
  41. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579-83 PMID: 4216019
  42. The length of the terminal repetition in adenovirus-2 DNA.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):3829-33 PMID: 4139703
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00571278
Published
1977-09-09
Pages
231-48
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00571278
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2