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PMID: 770435 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of the specialized transducing bacteriophages phi80dargF and lambdah80cI857 dargF: specific cleavage of arginine transducing deoxyribonucleic acid by the endonucleases EcoRI and SmaR.

Journal of bacteriology ·Vol. 126 ·No. 1 ·1976-04-00 ·Pages 487-500

James PM, Sens D, Natter W, Moore SK, James E

Abstract

The directed transposition of argF to the tonB locus of the Escherichia coli chromosome and the subsequent isolation of the specialized transducing phage phi80dargF is described. The structure of this phage has been has been determined. A hybrid lambdah80cI857dargF phage has been constructed. Deoxyribonucleic acid isolated from these and their parent bacteriophages has been specifically cleaved by the endonucleases EcoRI and SmaR; the unique deoxyribonucleic acid fragments thus obtained have been resolved and analyzed by electrophoresis in agarose gel.

MeSH Terms
Arginine/biosynthesis Chromosome Mapping Coliphages/analysis,isolation & purification DNA Restriction Enzymes/metabolism DNA, Viral/analysis,metabolism Escherichia coli/metabolism Genes Hybridization, Genetic Molecular Weight Plasmids Transduction, Genetic
Chemicals
DNA, Viral Arginine DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
James P M
Sens D
Natter W
Moore S K
James E
References (33)
33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.1.487-500.1976
Published
1976-04-00
Pages
487-500
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233305
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.1.487-500.1976 PMC Full Text View journal details
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