Abstract
Two genes for ornithinetranscarbamylase exist in strain Escherichia coli K-12, argI, at 85 min, and argF, at 7 min. In an attempt to compare the deoxyribonucleic acid material of these two genes, the lambda transducing phages carrying a portion of the argI region, lambda dvalS argI, lambda pvalS, and lambda dvalS pyrB, and of the argF region, lambda dargF, have been isolated. Their structure, including that of phi 80dargF previously isolated, was studied by the method of heteroduplex mapping. In this paper, the results of this mapping are reported.
MeSH Terms
Centrifugation, Density Gradient
Chromosome Mapping
Coliphages/analysis,isolation & purification
DNA Viruses
DNA, Viral/analysis
Escherichia coli/enzymology
Genes
Genetic Complementation Test
Lysogeny
Microscopy, Electron
Models, Biological
Mutation
Nucleic Acid Conformation
Ornithine Carbamoyltransferase/biosynthesis
Transduction, Genetic
Chemicals
DNA, Viral
Ornithine Carbamoyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kikuchi A
Elseviers D
Gorini L
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20 references, click to expand
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