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PMID: 1092666 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes.

Journal of bacteriology ·Vol. 122 ·No. 2 ·1975-05-00 ·Pages 727-42

Kikuchi A, Elseviers D, Gorini L

Abstract

Two genes for ornithinetranscarbamylase exist in strain Escherichia coli K-12, argI, at 85 min, and argF, at 7 min. In an attempt to compare the deoxyribonucleic acid material of these two genes, the lambda transducing phages carrying a portion of the argI region, lambda dvalS argI, lambda pvalS, and lambda dvalS pyrB, and of the argF region, lambda dargF, have been isolated. Their structure, including that of phi 80dargF previously isolated, was studied by the method of heteroduplex mapping. In this paper, the results of this mapping are reported.

MeSH Terms
Centrifugation, Density Gradient Chromosome Mapping Coliphages/analysis,isolation & purification DNA Viruses DNA, Viral/analysis Escherichia coli/enzymology Genes Genetic Complementation Test Lysogeny Microscopy, Electron Models, Biological Mutation Nucleic Acid Conformation Ornithine Carbamoyltransferase/biosynthesis Transduction, Genetic
Chemicals
DNA, Viral Ornithine Carbamoyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kikuchi A
Elseviers D
Gorini L
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1975-05-00
Pages
727-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246112
Subset
IM
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