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PMID: 9254674 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T-type Ca2+ current properties are not modified by Ca2+ channel beta subunit depletion in nodosus ganglion neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 17 ·1997-09-01 ·Pages 6621-8

Lambert RC, Maulet Y, Mouton J, Beattie R, Volsen S, De Waard M, Feltz A

Abstract

At the molecular level, our knowledge of the low voltage-activated Ca2+ channel (T-type) has made little progress. Using an antisense strategy, we investigated the possibility that the T-type channels have a structure similar to high voltage-activated Ca2+ channels. It is assumed that high voltage-activated channels are made of at least three components: a pore forming alpha1 subunit combined with a cytoplasmic modulatory beta subunit and a primarily extracellular alpha2delta subunit. We have examined the effect of transfecting cranial primary sensory neurons with generic anti-beta antisense oligonucleotides. We show that in this cell type, blocking expression of all known beta gene products does not affect T-type current, although it greatly decreases the current amplitude of high voltage-activated channels and modifies their voltage dependence. This suggests that beta subunits are likely not constitutive of T-type Ca2+ channels in this cell type.

MeSH Terms
Calcium/physiology Calcium Channel Blockers/pharmacology Calcium Channels/deficiency Cells, Cultured Electric Conductivity Immunohistochemistry Isomerism Neurons/metabolism,physiology Nodose Ganglion/cytology,metabolism,physiology Oligonucleotides, Antisense/pharmacology
Chemicals
Calcium Channel Blockers Calcium Channels Oligonucleotides, Antisense Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lambert R C
Laboratoire de Neurobiologie Cellulaire, UPR 9009 Centre National de la Recherche Scientifique, 67084 Strasbourg, France.
Maulet Y
Mouton J
Beattie R
Volsen S
De Waard M
Feltz A
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-09-01
Pages
6621-8
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6573151
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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