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PMID: 7679112 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and expression of a third calcium channel beta subunit.

The Journal of biological chemistry ·Vol. 268 ·No. 5 ·1993-02-15 ·Pages 3450-5

Castellano A, Wei X, Birnbaumer L, Perez-Reyes E

Abstract

The skeletal muscle dihydropyridine receptor/Ca2+ channel consists of five distinct subunits (alpha 1, alpha 2 delta, beta 1, and gamma). Homologous alpha 1, alpha 2 delta, and beta 2 subunits are expressed in heart and brain. The present study reports the cloning and expression of a third beta subunit, beta 3, which is expressed predominantly in brain. Its open reading frame encodes a protein with 484 amino acids with a predicted molecular mass of 54,571 Da. Coexpression of beta 3 with a cardiac alpha 1 in Xenopus oocytes induces similar changes in Ca2+ channel activity as beta 1 and beta 2, that is, it increases peak currents, modulates the voltage dependence of activation, and accelerates activation. In addition, beta 3 accelerates the rate of inactivation at positive test potentials.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Blotting, Northern Brain/physiology Calcium Channels/genetics,physiology Casein Kinases Cloning, Molecular DNA/genetics,isolation & purification Female Gene Library Heart/physiology Kinetics Macromolecular Substances Male Molecular Sequence Data Muscles/physiology Oligodeoxyribonucleotides Oocytes/physiology Organ Specificity Poly A/genetics,isolation & purification Polymerase Chain Reaction/methods Protein Kinase C/genetics Protein Kinases/genetics RNA/genetics,isolation & purification RNA, Messenger Rats Sequence Homology, Amino Acid Xenopus

Chemicals

Calcium Channels Macromolecular Substances Oligodeoxyribonucleotides RNA, Messenger Poly A RNA DNA Protein Kinases Casein Kinases Protein Kinase C

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Castellano A

Department of Molecular Physiology, Baylor College of Medicine, Houston, Texas 77030.

Wei X

Birnbaumer L

Perez-Reyes E

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1993-02-15

Pages

3450-5

Language

English

Region

United States

NLM ID

2985121R

Subset

Grants

NHLBI NIH HHS · HL-37044 · United States

NHLBI NIH HHS · HL-46702 · United States

External Links

PubMed source View journal details

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Cloning and expression of a third calcium channel beta subunit.

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