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PMID: 9223508 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sp1 binds to the precise locus of end processing within the terminal repeats of Epstein-Barr virus DNA.

Journal of virology ·Vol. 71 ·No. 8 ·1997-08-00 ·Pages 6136-43

Sun R, Spain TA, Lin SF, Miller G

Abstract

Interconversion between the linear genome of Epstein-Barr virus (EBV) present in virions and intracellular circular EBV DNA is a novel DNA recombination process. A previously characterized DNA binding activity called terminal repeat or tandem repeat binding protein (TRBP) was found to recognize several G-rich recombinogenic sequences in the EBV genome and in cellular DNA. TRBP was also found to be an autoantigen recognized by sera from certain patients with undifferentiated connective-tissue disorders. Here the transcription factor Sp1 has been identified as a component of TRBP and has been shown to be an autoantigen. Sp1 bound to recombination junctions of EBV DNA, such as those in the terminal repeats and in the large internal repeats, as well as to recombinogenic regions of cellular DNA, such as variable-number tandem repeats and switch regions of the immunoglobulin genes. We defined the ends of the linear EBV genome present in virions and showed that Sp1 binds to the sequence (GGGGTGGGGCATGGG) within EBV terminal repeats at the precise locus of interconversion of linear and circular viral DNA. Sp1 may be involved in DNA recombination.

MeSH Terms
Animals Base Sequence Binding Sites Callithrix Cells, Cultured DNA, Viral/metabolism Genome, Viral Herpesvirus 4, Human/genetics Humans Molecular Sequence Data Recombination, Genetic Repetitive Sequences, Nucleic Acid Sp1 Transcription Factor/metabolism
Chemicals
DNA, Viral Sp1 Transcription Factor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sun R
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520-8064, USA.
Spain T A
Lin S F
Miller G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.8.6136-6143.1997
Published
1997-08-00
Pages
6136-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191874
Subset
IM
Grants
NCI NIH HHS · CA16038 · United States
NIGMS NIH HHS · GM14637 · United States
NCI NIH HHS · T32CA09159 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.8.6136-6143.1997 PMC Full Text View journal details
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