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PMID: 8078938 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Autoantigenic proteins that bind recombinogenic sequences in Epstein-Barr virus and cellular DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 18 ·1994-08-30 ·Pages 8646-50

Sun R, Spain TA, Lin SF, Miller G

Abstract

We have identified conserved autoantigenic cellular proteins that bind to G-rich sequence motifs in recombinogenic regions of Epstein-Barr virus (EBV) DNA. This binding activity, called TRBP, recognizes the EBV terminal repeats, a locus responsible for interconversion of linear and circular EBV DNA. We found that TRBP also binds to EBV DNA sequences involved in deletion of EBNA2, a gene product required for immortalization. We show that TRBP binds sequences present in repetitive cellular DNA, such as variable-number tandem repeats (VNTR) and immunoglobulin heavy-chain class switch regions. We propose that EBV utilizes cellular DNA recombination systems to mediate several types of viral genome alterations. These findings may lead to an understanding of the mechanism of rearrangements of EBV DNA that are a central feature of the biology of the virus.

MeSH Terms
Animals Autoantigens/metabolism Base Sequence Binding Sites Callithrix DNA/metabolism DNA, Viral/metabolism DNA-Binding Proteins/immunology Herpesvirus 4, Human/genetics Humans Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Recombination, Genetic
Chemicals
Autoantigens DNA, Viral DNA-Binding Proteins Oligodeoxyribonucleotides DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sun R
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520-8064.
Spain T A
Lin S F
Miller G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.18.8646
Published
1994-08-30
Pages
8646-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44663
Subset
IM
Grants
NIAID NIH HHS · AI22959 · United States
NCI NIH HHS · CA12055 · United States
NCI NIH HHS · CA16038 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.18.8646 PMC Full Text View journal details
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