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PMID: 6787603 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of a rearranged gamma 1 chain gene and its implication to immunoglobulin class-switch mechanism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2437-41

Obata M, Kataoka T, Nakai S, Yamagishi H, Takahashi N, Yamawaki-Kataoka Y, Nikaido T, Shimizu A, Honjo T

Abstract

An expressed gene for gamma 1 chain of MC 101 myeloma was cloned from a phage library containing partial EcoRI digests of MC 101 DNA. The cloned DNA was analyzed by restriction enzyme cleavage, Southern blot hybridization, R-loop formation, and nucleotide sequence determination. The results indicate that the expressed gamma 1 chain gene comprises at least four germline DNA segments, namely a variable-region gene, a segment of the 5' flanking region of the mu chain gene (containing J regions), a segment of the 5' flanking region of the alpha chain gene, and the gamma 1 chain gene with its flanking regions. The presence of the alpha chain gene-flanking switch (S) region (S alpha region) at the 5' side of the gamma 1 chain gene-flanking region (S gamma 1 region) indicates that the heavy chain class switch may not be mediated by stepwise linear deletion along the order of the heavy chain constant-region genes (5'-mu-gamma 3-gamma 1-gamma 2b-gamma 2a-alpha-3'). We propose a siter-chromatid exchange model that explains class switch-associated deletion of heavy chain genes by unequal crossing-over events between sister chromatids.

MeSH Terms
Base Sequence Cell Line Gene Expression Regulation Genes Genetic Linkage Immunoglobulin Heavy Chains/genetics Immunoglobulin alpha-Chains/genetics Immunoglobulin gamma-Chains/genetics Myeloma Proteins/genetics Nucleic Acid Hybridization Recombination, Genetic
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin alpha-Chains Immunoglobulin gamma-Chains Myeloma Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Obata M
Kataoka T
Nakai S
Yamagishi H
Takahashi N
Yamawaki-Kataoka Y
Nikaido T
Shimizu A
Honjo T
References (37)
37 references, click to expand
  1. Distribution of allotypic specificities A1, A2, A14, and A15 among immunoglobulin G molecules.
    J Immunol. 1970 Mar;104(3):641-7 PMID: 5435789
  2. Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1581-5 PMID: 6262816
  3. Production of antibodies of identical idiotype but diverse immunoglobulin classes by cells derived from a single stimulated B cell.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1707-11 PMID: 1080568
  4. Genetic control of alpha chains of rabbit IgA: allotypic specificities on the variable and the constant regions.
    Contemp Top Mol Immunol. 1975;4:55-88 PMID: 53116
  5. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  6. Excision and duplication of su3+-transducing fragments carried by bacteriophage phi 80. I. Novel structure of phi 80sus2psu3+ DNA molecule.
    J Virol. 1976 Jun;18(3):1016-23 PMID: 1271527
  7. Quantitation of constant and variable region genes for mouse immunoglobulin lambda chains.
    Biochemistry. 1976 Jun 29;15(13):2780-5 PMID: 820372
  8. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  9. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  10. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  11. Evidence for noncontiguous variable and constant region genes in both germ line and myeloma DNA.
    Cell. 1978 Feb;13(2):319-27 PMID: 414842
  12. Organization of immunoglobulin heavy chain genes and allelic deletion model.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2140-4 PMID: 97665
  13. Internal structure of the nucleosome: DNA folding in the conserved 140-base-pair core particle.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:77-85 PMID: 277366
  14. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  15. The arrangement and rearrangement of antibody genes.
    Nature. 1978 Dec 21-28;276(5690):790-5 PMID: 103004
  16. Nucleotide sequence at the insertion sites of a kanamycin transposon.
    Nature. 1978 Dec 21-28;276(5690):845-7 PMID: 723960
  17. Cell differentiation in the presence of cytochalasin B: studies on the "switch" to IgG secretion after polyclonal B cell activation.
    J Immunol. 1979 Apr;122(4):1203-8 PMID: 376713
  18. Improved methods for the formation and stabilization of R-loops.
    Nucleic Acids Res. 1979 Jun 11;6(7):2499-317 PMID: 379821
  19. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene.
    Cell. 1979 Oct;18(2):559-68 PMID: 115593
  20. Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4240-4 PMID: 116231
  21. The role of gene deletion in the immunoglobulin heavy chain switch.
    Nature. 1980 Jan 24;283(5745):351-6 PMID: 6766206
  22. An immunoglobulin heavy-chain gene is formed by at least two recombinational events.
    Nature. 1980 Feb 21;283(5749):733-9 PMID: 6766532
  23. Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes.
    Cell. 1980 Jan;19(1):37-51 PMID: 6766810
  24. Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):919-23 PMID: 6767246
  25. Rearrangement of immunoglobulin heavy chain genes during B-lymphocyte development as revealed by studies of mouse plasmacytoma cells.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1422-6 PMID: 6769113
  26. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  27. Immunoglobulin gamma 1 heavy chain gene: structural gene sequences cloned in a bacterial plasmid.
    Gene. 1980 Apr;9(1-2):87-97 PMID: 6769752
  28. Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2909-13 PMID: 6771763
  29. Deletions in the constant region locus can account for switches in immunoglobulin heavy chain expression.
    Nature. 1980 Jun 12;285(5765):450-6 PMID: 6772957
  30. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  31. Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome.
    Nature. 1980 Aug 28;286(5776):850-3 PMID: 6774261
  32. DNA sequences mediating class switching in alpha-immunoglobulins.
    Science. 1980 Sep 19;209(4463):1360-5 PMID: 6774415
  33. Successive switching of antibody isotypes expressed within the lines of a B-cell clone.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5424-8 PMID: 6776537
  34. Nucleotide sequences of class-switch recombination region of the mouse immunoglobulin gamma 2b-chain gene.
    Gene. 1980 Oct;11(1-2):117-27 PMID: 7439686
  35. Complete nucleotide sequence of mouse immunoglobulin mu gene and comparison with other immunoglobulin heavy chain genes.
    Nucleic Acids Res. 1980 Sep 11;8(17):3933-45 PMID: 6255422
  36. Ordering of mouse immunoglobulin heavy chain genes by molecular cloning.
    Nature. 1981 Jan 15;289(5794):149-53 PMID: 6779206
  37. Association of allotypic specificities of group a with allotypic specificities A11 and A12 in rabbit immunoglobulin.
    Biochemistry. 1970 Apr 28;9(9):2028-32 PMID: 5442171
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2437
Published
1981-04-00
Pages
2437-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319361
Subset
IM
Databases
GENBANK
J00440, J00474, J00480, V01524
External Links
PubMed source DOI: 10.1073/pnas.78.4.2437 PMC Full Text View journal details
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