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PMID: 9199307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recombination signal sequence binding protein Jkappa is constitutively bound to the NF-kappaB site of the interleukin-6 promoter and acts as a negative regulatory factor.

Molecular and cellular biology ·Vol. 17 ·No. 7 ·1997-07-00 ·Pages 3733-43

Plaisance S, Vanden Berghe W, Boone E, Fiers W, Haegeman G

Abstract

Analysis by electrophoretic mobility shift assays (EMSA) of the different proteins associated with the kappaB sequence of the interleukin-6 (IL-6) promoter (IL6-kappaB) allowed us to detect a specific complex formed with the recombination signal sequence binding protein Jkappa (RBP-Jkappa). Single-base exchanges within the oligonucleotide sequence defined the critical base pairs involved in the interaction between RBP-Jkappa and the IL6-kappaB motif. Binding analysis suggests that the amount of RBP-Jkappa protein present in the nucleus is severalfold higher than the total amount of inducible NF-kappaB complexes but that the latter bind DNA with a 10-fold-higher affinity. A reporter gene study was performed to determine the functional implication of this binding; we found that the constitutive occupancy of the IL6-kappaB site by the RBP-Jkappa protein was responsible for the low basal levels of IL-6 promoter activity in L929sA fibrosarcoma cells and that RBP-Jkappa partially blocked access of NF-kappaB complexes to the IL-6 promoter. We propose that such a mechanism could be involved in the constitutive repression of the IL-6 gene under normal physiological conditions.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA-Binding Proteins/physiology Gene Expression Regulation Immunoglobulin J Recombination Signal Sequence-Binding Protein Interleukin-6/genetics Mice Mutagenesis, Site-Directed NF-kappa B/physiology Nuclear Proteins/metabolism Promoter Regions, Genetic Recombination, Genetic Repressor Proteins/physiology Transcription, Genetic Transcriptional Activation
Chemicals
DNA-Binding Proteins Immunoglobulin J Recombination Signal Sequence-Binding Protein Interleukin-6 NF-kappa B Nuclear Proteins Rbpj protein, mouse Repressor Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Plaisance S
Laboratory of Molecular Biology, Flanders Interuniversity Institute for Biotechnology and University of Ghent, Belgium.Stephen@lmb1.rug.ac .be
Vanden Berghe W
Boone E
Fiers W
Haegeman G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.7.3733
Email
belgium.stephen@lmb1.rug.ac
Published
1997-07-00
Pages
3733-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232225
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.7.3733 PMC Full Text View journal details
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