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PMID: 1312643 Published · ppublish English Journal Article

Mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells.

Journal of virology ·Vol. 66 ·No. 4 ·1992-04-00 ·Pages 2594-9

Robbins J, Blondel BJ, Gallahan D, Callahan R

Abstract

Expression of a 2.3-kb RNA species is induced in mammary tumors as a consequence of insertional mutagenesis at the int-3 locus by the mouse mammary tumor virus. The nucleotide sequence and biological activity of this mammary tumor-specific int-3 RNA species were determined. It contains an open reading frame which encodes a 57-kDa protein. The translated protein possesses six nearly contiguous 32-amino-acid repeats which are related to a similar motif in the Saccharomyces cerevisiae cdc-10-encoded cell cycle protein. In addition, the int-3 cdc-10 repeats are bounded by the PEST amino acid sequence motif which is commonly found in proteins having a rapid turnover and may represent sites for phosphorylation. The int-3 cdc-10 repeat sequences are 50% identical to a portion of the intracellular domain of the neurogenic Drosophila notch gene product. Activation of expression of a recombinant int-3 genomic DNA fragment encoding the 2.3-kb RNA species in HC11 mouse mammary epithelial cells in vitro induces anchorage-independent growth in soft agar.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cell Transformation, Viral/genetics DNA, Viral Epithelial Cells Insect Hormones/genetics Mammary Glands, Animal/cytology Mammary Tumor Virus, Mouse/genetics Membrane Proteins/genetics Mice Molecular Sequence Data Multigene Family Proto-Oncogene Proteins/genetics Receptor, Notch4 Receptors, Cell Surface/genetics Receptors, Notch Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Alignment Transcription, Genetic
Chemicals
DNA, Viral Insect Hormones Membrane Proteins Proto-Oncogene Proteins Receptor, Notch4 Receptors, Cell Surface Receptors, Notch Notch4 protein, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robbins J
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Maryland 20892.
Blondel B J
Gallahan D
Callahan R
References (40)
40 references, click to expand
  1. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family.
    Cell. 1987 Aug 28;50(5):729-37 PMID: 2957062
  2. Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila.
    Nature. 1987 Oct 15-21;329(6140):651-4 PMID: 2821408
  3. Mammary tumorigenesis in feral mice: identification of a new int locus in mouse mammary tumor virus (Czech II)-induced mammary tumors.
    J Virol. 1987 Jan;61(1):66-74 PMID: 3023708
  4. Prolactin regulation of beta-casein gene expression and of a cytosolic 120-kd protein in a cloned mouse mammary epithelial cell line.
    EMBO J. 1988 Jul;7(7):2089-95 PMID: 3416834
  5. Potential oncogene product related to growth factors.
    Nature. 1987 Apr 30-May 6;326(6116):833 PMID: 3574458
  6. Nucleotide sequence from the neurogenic locus notch implies a gene product that shares homology with proteins containing EGF-like repeats.
    Cell. 1985 Dec;43(3 Pt 2):567-81 PMID: 3935325
  7. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  8. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences.
    Nature. 1984 Feb 9-15;307(5951):521-7 PMID: 6320011
  9. Tumorigenesis by mouse mammary tumor virus: proviral activation of a cellular gene in the common integration region int-2.
    Cell. 1984 Jun;37(2):529-36 PMID: 6327073
  10. Epithelial mouse mammary cell line exhibiting normal morphogenesis in vivo and functional differentiation in vitro.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3756-60 PMID: 6587390
  11. A homolog of Drosophila Notch expressed during mammalian development.
    Development. 1991 Sep;113(1):199-205 PMID: 1764995
  12. Identification of Ets- and notch-related subunits in GA binding protein.
    Science. 1991 Aug 16;253(5021):789-92 PMID: 1876836
  13. Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins.
    Nature. 1990 Mar 1;344(6261):36-42 PMID: 2137557
  14. Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila.
    Cell. 1990 May 4;61(3):523-34 PMID: 2185893
  15. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs.
    Nature. 1990 Nov 1;348(6296):76-80 PMID: 2234062
  16. Xotch, the Xenopus homolog of Drosophila notch.
    Science. 1990 Sep 21;249(4975):1438-41 PMID: 2402639
  17. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  18. The scanning model for translation: an update.
    J Cell Biol. 1989 Feb;108(2):229-41 PMID: 2645293
  19. glp-1 and lin-12, genes implicated in distinct cell-cell interactions in C. elegans, encode similar transmembrane proteins.
    Cell. 1989 Aug 11;58(3):553-63 PMID: 2758466
  20. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
    Science. 1986 Oct 17;234(4774):364-8 PMID: 2876518
  21. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.
    Nucleic Acids Res. 1985 Mar 11;13(5):1431-42 PMID: 2987824
  22. The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless.
    Cell. 1987 Aug 14;50(4):649-57 PMID: 3111720
  23. Cell cycle control of the yeast HO gene: cis- and trans-acting regulators.
    Cell. 1987 Feb 13;48(3):389-97 PMID: 3542227
  24. Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 'start' gene.
    EMBO J. 1985 Feb;4(2):457-63 PMID: 4018034
  25. Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome.
    Cell. 1982 Nov;31(1):99-109 PMID: 6297757
  26. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  27. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity.
    Cell. 1991 Jun 28;65(7):1281-9 PMID: 1829648
  28. TAN-1, the human homolog of the Drosophila notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms.
    Cell. 1991 Aug 23;66(4):649-61 PMID: 1831692
  29. Convergence of Ets- and notch-related structural motifs in a heteromeric DNA binding complex.
    Science. 1991 Aug 16;253(5021):762-8 PMID: 1876833
  30. The Notch gene, adhesion, and developmental fate in the Drosophila embryo.
    New Biol. 1990 Jul;2(7):595-600 PMID: 2083251
  31. Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4519-23 PMID: 2162045
  32. The candidate proto-oncogene bcl-3 is related to genes implicated in cell lineage determination and cell cycle control.
    Cell. 1990 Mar 23;60(6):991-7 PMID: 2180580
  33. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.
    Cell. 1990 Sep 7;62(5):1007-18 PMID: 2203531
  34. The mouse int-2 gene exhibits basic fibroblast growth factor activity in a basic fibroblast growth factor-responsive cell line.
    Cell Growth Differ. 1990 Oct;1(10):463-72 PMID: 2278877
  35. The product of fem-1, a nematode sex-determining gene, contains a motif found in cell cycle control proteins and receptors for cell-cell interactions.
    Cell. 1990 Mar 23;60(6):981-90 PMID: 2317869
  36. Two proto-oncogenes implicated in mammary carcinogenesis, int-1 and int-2, are independently regulated during mouse development.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7806-10 PMID: 2429320
  37. The mouse homolog of the hst/k-FGF gene is adjacent to int-2 and is activated by proviral insertion in some virally induced mammary tumors.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5678-82 PMID: 2548184
  38. Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene.
    Cell. 1989 Apr 7;57(1):21-9 PMID: 2649246
  39. The yeast SWI4 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle-dependent transcription.
    Nature. 1989 Dec 14;342(6251):830-3 PMID: 2689885
  40. Genomic sequence of hst, a transforming gene encoding a protein homologous to fibroblast growth factors and the int-2-encoded protein.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7305-9 PMID: 2959959
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.4.2594-2599.1992
Published
1992-04-00
Pages
2594-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289064
Subset
IM
Databases
GENBANK
M80456
External Links
PubMed source DOI: 10.1128/JVI.66.4.2594-2599.1992 PMC Full Text View journal details
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