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PMID: 9174198 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of recalcitrance to ciprofloxacin and levofloxacin exhibited by Pseudomonas aeruginosa bofilms displaying rapid-transport characteristics.

Antimicrobial agents and chemotherapy ·Vol. 41 ·No. 6 ·1997-06-00 ·Pages 1352-8

Vrany JD, Stewart PS, Suci PA

Abstract

Attenuated total reflection Fourier transform infrared spectroscopy was used to measure transport of the fluoroquinolones (FQs) ciprofloxacin and levofloxacin into Pseudomonas aeruginosa biofilms. Biofilms were exposed to each FQ at dose levels of 100, 250, and 500 microg/ml for 30 min. A mathematical transport model was used to extract the diffusion coefficient, binding site density, and adsorption and desorption rates for each experiment. Recalcitrance of the biofilms toward each FQ was evaluated by comparison of efficacies with planktonic bacteria. By this criterion, biofilms were found to exhibit more recalcitrance toward levofloxacin than ciprofloxacin under the experimental conditions. These results cannot be explained by the more hindered transport of levofloxacin, implicating the domination of physiological factors.

MeSH Terms
Anti-Infective Agents/pharmacokinetics Biofilms/drug effects Biological Transport Ciprofloxacin/pharmacokinetics Levofloxacin Ofloxacin/pharmacokinetics Pseudomonas aeruginosa/metabolism,physiology Spectroscopy, Fourier Transform Infrared
Chemicals
Anti-Infective Agents Ciprofloxacin Levofloxacin Ofloxacin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vrany J D
Center for Biofilm Engineering, Montana State University, Bozeman 59717, USA.
Stewart P S
Suci P A
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.41.6.1352
Published
1997-06-00
Pages
1352-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163914
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.41.6.1352 PMC Full Text View journal details
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