Home LiteratureArticle Details

PMID: 2511804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of biofilm bacteria with antibiotics in a novel in vitro chemostat system.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 10 ·1989-10-00 ·Pages 1824-6

Anwar H, van Biesen T, Dasgupta M, Lam K, Costerton JW

Abstract

Pseudomonas aeruginosa was cultivated at low growth rates under iron-limiting conditions on acrylic tiles. Biofilm cells exhibited increased tobramycin resistance compared with that of planktonic cells, and in old biofilms were more resistant than were cells in young biofilms. However, on suspension of the biofilm bacteria, glycocalyx-mediated resistance was lost.

MeSH Terms

Anti-Bacterial Agents/pharmacology Cell Membrane/drug effects,physiology,ultrastructure Drug Resistance, Microbial Microbial Sensitivity Tests Pseudomonas aeruginosa/drug effects,growth & development,ultrastructure Tobramycin/pharmacology

Chemicals

Anti-Bacterial Agents Tobramycin

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Anwar H

Department of Microbiology, University of Alberta, Edmonton, Canada.

van Biesen T

Dasgupta M

Lam K

Costerton J W

References (19)

19 references, click to expand

Microbial surfaces in relation to pathogenicity.
Bacteriol Rev. 1977 Jun;41(2):475-500 PMID: 407898
Antibody response to outer-membrane antigens of Pseudomonas aeruginosa in human burn wound infection.
J Med Microbiol. 1988 Nov;27(3):179-90 PMID: 3143013
Sequelae and management of urinary infection in the patient requiring chronic catheterization.
J Urol. 1981 Jan;125(1):1-8 PMID: 7007662
The role of the microcolony mode of growth in the pathogenesis of Pseudomonas aeruginosa infections.
Rev Infect Dis. 1983 Nov-Dec;5 Suppl 5:S867-73 PMID: 6419312
Influence of nutrient limitation of growth on stability and production of virulence factors of mucoid and nonmucoid strains of Pseudomonas aeruginosa.
Rev Infect Dis. 1983 Nov-Dec;5 Suppl 5:S880-8 PMID: 6419314
Iron withholding: a defense against infection and neoplasia.
Physiol Rev. 1984 Jan;64(1):65-102 PMID: 6420813
Electron microscopic study of an infected Foley catheter.
Can J Surg. 1985 Jan;28(1):50-1, 54 PMID: 3971223
In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions.
Infect Immun. 1985 Apr;48(1):35-9 PMID: 3980092
Effect of subinhibitory concentrations of cephalosporins on surface properties and siderophore production in iron-depleted Klebsiella pneumoniae.
Antimicrob Agents Chemother. 1985 Feb;27(2):220-3 PMID: 3157345
Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material.
Antimicrob Agents Chemother. 1985 Apr;27(4):619-24 PMID: 3923925
Culture of the surfaces of urinary catheters to sample urethral flora and study the effect of antimicrobial therapy.
J Clin Microbiol. 1985 Jun;21(6):902-8 PMID: 4008620
The influence of environment on envelope properties affecting survival of bacteria in infections.
Annu Rev Microbiol. 1985;39:527-56 PMID: 3904611
Microbial film formation: dental plaque deposition on acrylic tiles using continuous culture techniques.
J Appl Bacteriol. 1987 Feb;62(2):129-38 PMID: 3571036
Media for study of growth kinetics and envelope properties of iron-deprived bacteria.
J Clin Microbiol. 1987 May;25(5):849-55 PMID: 3108311
Bacterial biofilms in nature and disease.
Annu Rev Microbiol. 1987;41:435-64 PMID: 3318676
Method of evaluating effects of antibiotics on bacterial biofilm.
Antimicrob Agents Chemother. 1987 Oct;31(10):1502-6 PMID: 3435100
Influence of cephalosporins and iron on surface protein antigens of Klebsiella pneumoniae in vivo.
Antimicrob Agents Chemother. 1988 Mar;32(3):364-8 PMID: 3284461
Structure and plasticity at various organization levels in the bacterial cell.
Can J Microbiol. 1988 Apr;34(4):513-21 PMID: 3052758
Iron and infection.
Microbiol Rev. 1978 Mar;42(1):45-66 PMID: 379572

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.33.10.1824

Published

1989-10-00

Pages

1824-6

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC172765

Subset

External Links

PubMed source DOI: 10.1128/AAC.33.10.1824 PMC Full Text View journal details

Analysis Services

Interaction of biofilm bacteria with antibiotics in a novel in vitro chemostat system.

Contact

WeChat Official Account