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PMID: 9171092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chloroplast endoribonuclease p54 involved in RNA 3'-end processing is regulated by phosphorylation and redox state.

Nucleic acids research ·Vol. 25 ·No. 12 ·1997-06-15 ·Pages 2403-8

Liere K, Link G

Abstract

Chloroplast RNA-binding protein p54 is an endoribonuclease required for 3'end-processing of plastid precursor transcripts. We find that purified p54 can serve as a phosphate acceptor for protein kinases in vitro. Both the processing and RNA-binding activities of p54 are enhanced by phosphorylation and decreased by dephosphorylation. In addition, the enzyme is activated by the oxidized form of glutathione and inhibited by the reduced form, whereas other redox reagents that were tested showed no effect. Kinase treatment of p54 prior to oxidation by glutathione resulted in highest levels of activation, suggesting that phosphorylation and redox state act together to control p54 activity in vitro and possibly also in vivo.

MeSH Terms
Chloroplast Proteins Chloroplasts/enzymology Endoribonucleases/isolation & purification,metabolism Mustard Plant Oxidation-Reduction Phosphorylation Plants, Medicinal Plastids Protein Processing, Post-Translational RNA Processing, Post-Transcriptional RNA, Plant/metabolism RNA-Binding Proteins/isolation & purification,metabolism Substrate Specificity Transcription, Genetic
Chemicals
Chloroplast Proteins RNA, Plant RNA-Binding Proteins Endoribonucleases chloroplast RNA-binding protein p54
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liere K
Plant Cell Physiology and Molecular Biology, University of Bochum, D-44780 Bochum, Germany.
Link G
References (45)
45 references, click to expand
  1. Translational regulation of gene expression in chloroplasts and mitochondria.
    Annu Rev Genet. 1994;28:71-93 PMID: 7893142
  2. An AU-rich element in the 3' untranslated region of the spinach chloroplast petD gene participates in sequence-specific RNA-protein complex formation.
    Mol Cell Biol. 1995 Apr;15(4):2010-18 PMID: 7891696
  3. Modulation of transcription factor NF kappa B activity by intracellular glutathione levels and by variations of the extracellular cysteine supply.
    FASEB J. 1995 Feb;9(2):246-52 PMID: 7781927
  4. Phosphorylation of a chloroplast RNA-binding protein changes its affinity to RNA.
    Nucleic Acids Res. 1995 Jul 11;23(13):2506-11 PMID: 7630729
  5. Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco.
    Plant J. 1995 Aug;8(2):167-75 PMID: 7670502
  6. Identification and characterization of the Arabidopsis thaliana chloroplast DNA region containing the genes psbA, trnH and rps19'.
    Curr Genet. 1995 Jul;28(2):128-30 PMID: 8590463
  7. Glutathione is required for maximal transcription of the cobalamin biosynthetic and 1,2-propanediol utilization (cob/pdu) regulon and for the catabolism of ethanolamine, 1,2-propanediol, and propionate in Salmonella typhimurium LT2.
    J Bacteriol. 1995 Oct;177(19):5434-9 PMID: 7559326
  8. Translational regulation of chloroplast genes. Proteins binding to the 5'-untranslated regions of chloroplast mRNAs in Chlamydomonas reinhardtii.
    J Biol Chem. 1996 Jan 19;271(3):1486-97 PMID: 8576143
  9. Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.
    EMBO J. 1996 Mar 1;15(5):1132-41 PMID: 8605883
  10. Cis-acting elements and trans-acting factors for accurate translation of chloroplast psbA mRNAs: development of an in vitro translation system from tobacco chloroplasts.
    EMBO J. 1996 Apr 1;15(7):1687-95 PMID: 8612593
  11. Glutathione is an essential metabolite required for resistance to oxidative stress in the yeast Saccharomyces cerevisiae.
    Curr Genet. 1996 May;29(6):511-5 PMID: 8662189
  12. CSP41, a sequence-specific chloroplast mRNA binding protein, is an endoribonuclease.
    Plant Cell. 1996 Aug;8(8):1409-20 PMID: 8776902
  13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  14. Silver staining methods for polyacrylamide gel electrophoresis.
    Methods Enzymol. 1983;96:230-9 PMID: 6197605
  15. Chloroplast gene expression: how plants turn their plastids on.
    Cell. 1989 Jan 27;56(2):161-70 PMID: 2643471
  16. mRNA decay: finding the right targets.
    Cell. 1989 Apr 7;57(1):9-10 PMID: 2467747
  17. Mechanisms of mRNA decay in bacteria: a perspective.
    Gene. 1988 Dec 10;72(1-2):15-23 PMID: 3072246
  18. Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii.
    EMBO J. 1989 Apr;8(4):1013-21 PMID: 2663467
  19. Function of plastid mRNA 3' inverted repeats. RNA stabilization and gene-specific protein binding.
    J Biol Chem. 1989 Nov 5;264(31):18742-50 PMID: 2478550
  20. Interaction of a 3' RNA region of the mustard trnK gene with chloroplast proteins.
    Nucleic Acids Res. 1989 Dec 11;17(23):9637-48 PMID: 2481265
  21. Characterisation of human and murine snRNP proteins by two-dimensional gel electrophoresis and phosphopeptide analysis of U1-specific 70K protein variants.
    Nucleic Acids Res. 1990 Aug 11;18(15):4427-38 PMID: 2143816
  22. Redox regulation of fos and jun DNA-binding activity in vitro.
    Science. 1990 Sep 7;249(4973):1157-61 PMID: 2118682
  23. Chloroplast mRNA 3' end processing requires a nuclear-encoded RNA-binding protein.
    EMBO J. 1991 Jun;10(6):1493-502 PMID: 2026146
  24. Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1.
    EMBO J. 1991 Aug;10(8):2247-58 PMID: 2065663
  25. Specific binding of chloroplast proteins in vitro to the 3' untranslated region of spinach chloroplast petD mRNA.
    Mol Cell Biol. 1991 Sep;11(9):4380-8 PMID: 1908552
  26. RNA-protein interactions at transcript 3' ends and evidence for trnK-psbA cotranscription in mustard chloroplasts.
    Mol Gen Genet. 1991 Aug;228(1-2):89-96 PMID: 1715978
  27. Specific ribonuclease activities in spinach chloroplasts promote mRNA maturation and degradation.
    J Biol Chem. 1991 Dec 15;266(35):24205-11 PMID: 1721067
  28. Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
    EMBO J. 1991 Dec;10(13):3993-4001 PMID: 1721866
  29. SR proteins: a conserved family of pre-mRNA splicing factors.
    Genes Dev. 1992 May;6(5):837-47 PMID: 1577277
  30. The chloroplast genome.
    Plant Mol Biol. 1992 May;19(1):149-68 PMID: 1600166
  31. Post-transcriptional steps in the expression of chloroplast genes.
    Annu Rev Cell Biol. 1992;8:1-28 PMID: 1282351
  32. Phosphorylation of human hnRNP protein A1 abrogates in vitro strand annealing activity.
    Nucleic Acids Res. 1993 Feb 25;21(4):949-55 PMID: 8451194
  33. A functional promoter shift of a chloroplast gene: a transcriptional fusion between a novel psbA gene copy and the trnK (UUU) gene in Pinus contorta.
    Plant J. 1992 Nov;2(6):875-86 PMID: 1302639
  34. Phosphorylation and dephosphorylation affect functional characteristics of chloroplast and etioplast transcription systems from mustard (Sinapis alba L.).
    EMBO J. 1993 May;12(5):1745-53 PMID: 8491168
  35. Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors.
    Nucleic Acids Res. 1993 Jun 25;21(12):2815-22 PMID: 8332490
  36. Serine/threonine phosphorylation regulates binding of C hnRNP proteins to pre-mRNA.
    Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7764-8 PMID: 8356082
  37. The 54 kDa RNA-binding protein from mustard chloroplasts mediates endonucleolytic transcript 3' end formation in vitro.
    Plant J. 1993 Apr;3(4):537-44 PMID: 8220460
  38. Biochemical characterization of a 34 kDa ribonucleoprotein (p34) purified from the spinach chloroplast fraction as an effective phosphate acceptor for casein kinase II.
    FEBS Lett. 1993 Dec 6;335(2):176-80 PMID: 8253191
  39. Evolutionary clustering and functional similarity of RNA-binding proteins.
    FEBS Lett. 1993 Dec 6;335(2):289-93 PMID: 8253214
  40. ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation.
    EMBO J. 1994 May 1;13(9):2227-35 PMID: 8187775
  41. Dynamic regulation of chloroplast transcription.
    Plant Physiol. 1993 Oct;103(2):309-13 PMID: 8029331
  42. A nuclear mutation in maize blocks the processing and translation of several chloroplast mRNAs and provides evidence for the differential translation of alternative mRNA forms.
    EMBO J. 1994 Jul 1;13(13):3170-81 PMID: 8039510
  43. Determinants for stability of the chloroplast psbD RNA are located within its short leader region in Chlamydomonas reinhardtii.
    EMBO J. 1994 Jul 1;13(13):3182-91 PMID: 8039511
  44. Light-regulated translation of chloroplast messenger RNAs through redox potential.
    Science. 1994 Dec 9;266(5191):1717-9 PMID: 7992056
  45. Degradation of mRNA in eukaryotes.
    Cell. 1995 Apr 21;81(2):179-83 PMID: 7736570
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.12.2403
Published
1997-06-15
Pages
2403-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146767
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/25.12.2403 PMC Full Text View journal details
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