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PMID: 2065663 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1.

The EMBO journal ·Vol. 10 ·No. 8 ·1991-08-00 ·Pages 2247-58

Schreck R, Rieber P, Baeuerle PA

Abstract

Hydrogen peroxide and oxygen radicals are agents commonly produced during inflammatory processes. In this study, we show that micromolar concentrations of H2O2 can induce the expression and replication of HIV-1 in a human T cell line. The effect is mediated by the NF-kappa B transcription factor which is potently and rapidly activated by an H2O2 treatment of cells from its inactive cytoplasmic form. N-acetyl-L-cysteine (NAC), a well characterized antioxidant which counteracts the effects of reactive oxygen intermediates (ROI) in living cells, prevented the activation of NF-kappa B by H2O2. NAC and other thiol compounds also blocked the activation of NF-kappa B by cycloheximide, double-stranded RNA, calcium ionophore, TNF-alpha, active phorbol ester, interleukin-1, lipopolysaccharide and lectin. This suggests that diverse agents thought to activate NF-kappa B by distinct intracellular pathways might all act through a common mechanism involving the synthesis of ROI. ROI appear to serve as messengers mediating directly or indirectly the release of the inhibitory subunit I kappa B from NF-kappa B.

MeSH Terms
Base Sequence Cell Line Cycloheximide/pharmacology DNA/metabolism Enhancer Elements, Genetic Free Radicals HIV-1/genetics,physiology Humans Hydrogen Peroxide/chemistry Interleukin-1/metabolism Lectins/metabolism Lipopolysaccharides/metabolism Molecular Sequence Data NF-kappa B/metabolism Oxygen/chemistry Promoter Regions, Genetic Protein Kinase C/metabolism RNA, Double-Stranded/metabolism T-Lymphocytes/metabolism Tetradecanoylphorbol Acetate/pharmacology Tumor Necrosis Factor-alpha/metabolism Virus Replication
Chemicals
Free Radicals Interleukin-1 Lectins Lipopolysaccharides NF-kappa B RNA, Double-Stranded Tumor Necrosis Factor-alpha DNA Cycloheximide Hydrogen Peroxide Protein Kinase C Tetradecanoylphorbol Acetate Oxygen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schreck R
Laboratorium für Molekulare Biologie, Ludwig-Maximilians-Universität München, FRG.
Rieber P
Baeuerle P A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-08-00
Pages
2247-58
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452914
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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