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PMID: 1908552 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Specific binding of chloroplast proteins in vitro to the 3' untranslated region of spinach chloroplast petD mRNA.

Molecular and cellular biology ·Vol. 11 ·No. 9 ·1991-09-00 ·Pages 4380-8

Chen HC, Stern DB

Abstract

A detailed analysis of RNA-protein complex formation in the 3' untranslated region of spinach chloroplast petD mRNA has been carried out. Five chloroplast proteins that interact with petD RNA in this region, which contains an inverted repeat sequence capable of forming a hairpin structure, have been identified. A 33-kDa protein recognizes specifically the double-stranded stem of the hairpin structure; mutations that disrupt base pairing at the base of the stem reduce or eliminate protein binding. A 57-kDa protein recognizes specifically an AU-rich sequence motif that is highly conserved in petD genes of different higher plant species. The 57-kDa protein and possibly the 33-kDa protein form stable complexes with petD RNA in vitro and may interact with each other. In addition, their interaction with petD RNA is highly sensitive to heparin. The three other proteins, of 100, 32, and 28 kDa, display little sequence or structural binding specificity apart from their preference for uridine-rich sequences. They also interact with the 3' untranslated regions of other chloroplast RNAs such as those of psbA and rbcL. The functions of these proteins in the regulation of petD gene expression, including possible roles in transcription termination and RNA stability, are discussed.

Related Genes
petD
MeSH Terms
Base Composition Base Sequence Binding Sites Binding, Competitive Carrier Proteins/metabolism Chloroplasts/metabolism Heparin/pharmacology Introns Molecular Sequence Data Nucleic Acid Conformation Plant Proteins/metabolism Plants/genetics RNA, Messenger/metabolism,radiation effects RNA-Binding Proteins Transcription, Genetic Ultraviolet Rays
Chemicals
Carrier Proteins Plant Proteins RNA, Messenger RNA-Binding Proteins Heparin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chen H C
Boyce Thompson Institute for Plant Research, Ithaca, New York 14853-1801.
Stern D B
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.9.4380-4388.1991
Published
1991-09-00
Pages
4380-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361300
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.9.4380-4388.1991 PMC Full Text View journal details
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