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PMID: 2140872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.

Nature ·Vol. 345 ·No. 6275 ·1990-06-07 ·Pages 502-6

Scherly D, Boelens W, Dathan NA, van Venrooij WJ, Mattaj IW

Abstract

The basis of the specificity of interaction of U1 and U2 small nuclear (sn)RNAs and their cognate binding proteins, U1A and U2B'', has been examined. The U1A protein recognizes U1 snRNA on its own, whereas U2B'' binds specifically to U2 snRNA only in the presence of a second protein, U2A'. Exchange of two nucleotides between the two RNAs or of eight amino acids between the two proteins reverses binding specificity.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Binding Sites Carrier Proteins/metabolism,ultrastructure DNA Mutational Analysis Hydrogen Bonding Molecular Sequence Data Nucleic Acid Conformation RNA, Small Nuclear/metabolism,ultrastructure RNA-Binding Proteins Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Structure-Activity Relationship Xenopus laevis

Chemicals

Carrier Proteins RNA, Small Nuclear RNA-Binding Proteins Ribonucleoproteins Ribonucleoproteins, Small Nuclear

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Scherly D

European Molecular Biology Laboratory, Heidelberg, FRG.

Boelens W

Dathan N A

van Venrooij W J

Mattaj I W

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/345502a0

Published

1990-06-07

Pages

502-6

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/345502a0 View journal details

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Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.

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