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PMID: 9092617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The ZiN/POZ domain of ZF5 is required for both transcriptional activation and repression.

Nucleic acids research ·Vol. 25 ·No. 6 ·1997-03-15 ·Pages 1108-16

Kaplan J, Calame K

Abstract

ZF5 is a ubiquitously expressed protein originally identified by its ability to bind and repress the murine c-myc promoter. It contains five C-terminal zinc fingers and a conserved N-terminal ZiN/POZ domain. This motif, found in a growing number of zinc finger proteins, can inhibit DNA binding and mediate dimerization [Bardwell, V.J. and Treisman,R. (1994) Genes Dev., 8,1664-1677]. In the current study, a cyclic amplification and selection of targets (CAST) protocol detected preferred ZF5 binding sites which are highly GC-rich. Binding to these sites by ZF5 depended upon the zinc fingers and was enhanced when the ZiN/POZ domain was removed. Using transient cotransfection assays, ZF5 was shown to activate the HIV-1 LTR and repress the beta-actin promoter. The ZiN/POZ domain was shown to mediate ZF5-dependent transcriptional activation and repression. From these data, we conclude that ZF5 can both activate and repress in the context of different natural promoters and that its ZiN/POZ domain can affect two functions; DNA binding and transcriptional modulation.

MeSH Terms
3T3 Cells Actins/genetics Amino Acid Sequence Animals Base Sequence Binding Sites Cloning, Molecular Consensus Sequence Conserved Sequence DNA/chemistry,metabolism DNA Footprinting DNA-Binding Proteins/chemistry,metabolism HIV Long Terminal Repeat Humans Kruppel-Like Transcription Factors Mice Molecular Sequence Data Oligodeoxyribonucleotides/metabolism Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins/chemistry,metabolism Repressor Proteins/chemistry,metabolism Transcription Factors/chemistry,metabolism Transfection Zinc Fingers
Chemicals
Actins DNA-Binding Proteins Kruppel-Like Transcription Factors Oligodeoxyribonucleotides Recombinant Fusion Proteins Repressor Proteins Transcription Factors ZBTB14 protien, human Zfp161 protein, mouse DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaplan J
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Calame K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.6.1108
Published
1997-03-15
Pages
1108-16
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146578
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/25.6.1108 PMC Full Text View journal details
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