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PMID: 3313385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A plasmacytoma-specific factor binds the c-myc promoter region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 20 ·1987-10-00 ·Pages 7031-5

Kakkis E, Calame K

Abstract

We used an electrophoretic mobility-shift assay to study proteins that bind to sequences in the 5' flanking region of the murine c-myc gene. By comparing the DNA-protein complexes formed with extracts from cells representing earlier stages of B-cell development with those from plasmacytomas, we identified a plasmacytoma-specific protein that binds to a region within the c-myc promoter. Five other regions of this promoter show extensive sequence-specific binding, but the binding is not clearly B-cell stage-specific. Methylation-interference and o-phenanthroline/copper-protection experiments identified a single plasmacytoma-specific protein binding site 290 base pairs 5' of the transcription start site P1. Homologues of a core sequence, d(AGAAAGGGAAAGGA), within the 25-base-pair binding site are found at three additional sites in the murine c-myc locus. The plasmacytoma-specific occurrence of this protein suggests that it may play a role in the transcriptional repression of the normal c-myc gene observed in plasmacytomas.

MeSH Terms
Animals B-Lymphocytes/pathology Base Sequence Binding Sites Cell Differentiation DNA-Binding Proteins/isolation & purification,metabolism Humans Mice Neoplasm Proteins/isolation & purification,metabolism Plasmacytoma/metabolism Promoter Regions, Genetic Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes Sequence Homology, Nucleic Acid
Chemicals
DNA-Binding Proteins Neoplasm Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kakkis E
Department of Biological Chemistry, University of California, Los Angeles 90024.
Calame K
References (35)
35 references, click to expand
  1. Intragenic pausing and anti-sense transcription within the murine c-myc locus.
    EMBO J. 1986 Nov;5(11):2859-65 PMID: 3024965
  2. Target sequences for cis-acting regulation within the dual promoter of the human c-myc gene.
    Mol Cell Biol. 1987 Apr;7(4):1393-400 PMID: 3037316
  3. Transcriptionally active DNA region that rearranges frequently in murine lymphoid tumors.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6966-70 PMID: 6294668
  4. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  5. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas.
    Nature. 1983 Jun 2-8;303(5916):401-6 PMID: 6304522
  6. Translocations among antibody genes in human cancer.
    Science. 1983 Nov 18;222(4625):765-71 PMID: 6356357
  7. Interchromosomal recombination of the cellular oncogene c-myc with the immunoglobulin heavy chain locus in murine plasmacytomas is a reciprocal exchange.
    EMBO J. 1983;2(5):697-703 PMID: 6416831
  8. Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
    EMBO J. 1983;2(12):2375-83 PMID: 6321164
  9. Transcriptional activation of the translocated c-myc oncogene in mouse plasmacytomas: similar RNA levels in tumor and proliferating normal cells.
    Cell. 1984 Jun;37(2):521-8 PMID: 6327072
  10. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells.
    Nature. 1984 Aug 16-22;310(5978):592-4 PMID: 6462247
  11. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes.
    Cell. 1984 Oct;38(3):627-37 PMID: 6488314
  12. Fibroblast lines expressing activated c-myc oncogenes are tumorigenic in nude mice and syngeneic animals.
    Cell. 1984 Dec;39(2 Pt 1):339-48 PMID: 6498938
  13. Translocation affects normal c-myc promoter usage and activates fifteen cryptic c-myc transcription starts in plasmacytoma M603.
    Nucleic Acids Res. 1984 Dec 11;12(23):8987-9007 PMID: 6096815
  14. Transposition of the immunoglobulin heavy chain enhancer to the myc oncogene in a murine plasmacytoma.
    Cell. 1985 Jan;40(1):71-9 PMID: 2981633
  15. Suppression of the normal mouse c-myc oncogene in human lymphoma cells.
    Nature. 1985 Feb 7-13;313(6002):493-5 PMID: 3838203
  16. Coexpression of translocated and normal c-myc oncogenes in hybrids between Daudi and lymphoblastoid cells.
    Science. 1985 Mar 8;227(4691):1235-8 PMID: 3856319
  17. Chromosome translocation activates heterogeneously initiated, bipolar transcription of a mouse c-myc gene.
    EMBO J. 1985 Mar;4(3):667-74 PMID: 3924591
  18. The c-myc oncogene driven by immunoglobulin enhancers induces lymphoid malignancy in transgenic mice.
    Nature. 1985 Dec 12-18;318(6046):533-8 PMID: 3906410
  19. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  20. Chromatin structure of the murine c-myc locus: implications for the regulation of normal and chromosomally translocated genes.
    EMBO J. 1985 Dec 1;4(12):3195-202 PMID: 3004939
  21. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment.
    Nature. 1986 Apr 24-30;320(6064):760-3 PMID: 3458027
  22. Chromosomal breakpoints and structural alterations of the c-myc locus differ in endemic and sporadic forms of Burkitt lymphoma.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2984-8 PMID: 3458257
  23. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells.
    Nature. 1986 Jun 12-18;321(6071):702-6 PMID: 3520340
  24. Autocrine beta-related interferon controls c-myc suppression and growth arrest during hematopoietic cell differentiation.
    Cell. 1986 Jul 4;46(1):31-40 PMID: 2941150
  25. A negative transcriptional control element located upstream of the murine c-myc gene.
    EMBO J. 1986 May;5(5):899-904 PMID: 3013622
  26. Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation.
    Nature. 1986 Aug 28-Sep 3;322(6082):848-50 PMID: 3528863
  27. Trans-acting elements modulate expression of the human c-myc gene in Burkitt lymphoma cells.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7918-22 PMID: 3464006
  28. Participation of c-myc protein in DNA synthesis of human cells.
    Science. 1986 Oct 24;234(4775):467-70 PMID: 3532322
  29. Activation of cellular oncogenes in hemopoietic cells by chromosome translocation.
    Adv Cancer Res. 1986;47:189-234 PMID: 3096089
  30. An active chromatin structure acquired by translocated c-myc genes.
    Mol Cell Biol. 1986 Apr;6(4):1357-61 PMID: 3023885
  31. Sequences involved in accurate and efficient transcription of human c-myc genes microinjected into frog oocytes.
    Mol Cell Biol. 1986 Nov;6(11):4093-8 PMID: 3025632
  32. Binding in vitro of multiple cellular proteins to immunoglobulin heavy-chain enhancer DNA.
    Mol Cell Biol. 1986 Dec;6(12):4168-78 PMID: 3025641
  33. Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression.
    Mol Cell Biol. 1986 Dec;6(12):4305-16 PMID: 3025651
  34. The first exon of the c-myc proto-oncogene contains a novel positive control element.
    EMBO J. 1986 Dec 20;5(13):3553-62 PMID: 3030732
  35. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.20.7031
Published
1987-10-00
Pages
7031-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299223
Subset
IM
Grants
NCI NIH HHS · CA38571 · United States
NIGMS NIH HHS · GM29361 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.20.7031 PMC Full Text View journal details
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