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PMID: 3004939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Chromatin structure of the murine c-myc locus: implications for the regulation of normal and chromosomally translocated genes.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3195-202

Fahrlander PD, Piechaczyk M, Marcu KB

Abstract

To assess possible alterations of c-myc transcriptional control in murine B-cell tumors, we have investigated the pattern of DNaseI hypersensitive sites in the gene's putative regulatory region and within the gene in a variety of genomic contexts. A number of such sites were found in several cell types, but none of these was detectable in a gene which was shown to be transcriptionally silent by the criterion of elongation of nascent transcripts in isolated nuclei. These results differ from those of a previous study, in which a DNaseI-hypersensitive site approximately 2 kb upstream of the gene was proposed to be associated with negative regulation of c-myc transcription in human cells. An analysis of DNA sequences presented here reveals that this region is highly homologous between mouse and human, suggesting that these upstream hypersensitive sites do not reflect species-specific regulatory elements. We also present data indicating that this hypersensitive site distinguishes the c-myc alleles in translocation-positive plasma cell tumors which lack c-myc rearrangement. Furthermore, we report the existence of hypersensitive sites within the gene. One of these appears to be associated with cryptic promoters that are employed only when the normal promoters are lost as a consequence of chromosome translocation. These results are discussed in the context of c-myc translocation and gene breakage and with respect to possible stage-specific regulation of the gene's transcriptional competence.

MeSH Terms
Animals Base Sequence Burkitt Lymphoma/genetics Cell Line Chromatin/ultrastructure DNA Restriction Enzymes Deoxyribonuclease I Humans Mice Nucleic Acid Hybridization Oncogenes Transcription, Genetic Translocation, Genetic
Chemicals
Chromatin DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fahrlander P D
Piechaczyk M
Marcu K B
References (51)
51 references, click to expand
  1. Post-transcriptional regulation of glyceraldehyde-3-phosphate-dehydrogenase gene expression in rat tissues.
    Nucleic Acids Res. 1984 Sep 25;12(18):6951-63 PMID: 6548307
  2. Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element.
    Cell. 1984 Aug;38(1):29-38 PMID: 6088072
  3. Viral integration near c-myc in 10-20% of mcf 247-induced AKR lymphomas.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6808-11 PMID: 6093121
  4. Transposition of the immunoglobulin heavy chain enhancer to the myc oncogene in a murine plasmacytoma.
    Cell. 1985 Jan;40(1):71-9 PMID: 2981633
  5. Chromatin structure around the c-myc gene in Burkitt lymphomas with upstream and downstream translocation points.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1984-8 PMID: 3856876
  6. A high affinity topoisomerase I binding sequence is clustered at DNAase I hypersensitive sites in Tetrahymena R-chromatin.
    Cell. 1985 Jun;41(2):541-51 PMID: 2985282
  7. Evolution of tumours and the impact of molecular oncology.
    Nature. 1985 May 16-22;315(6016):190-5 PMID: 2987696
  8. Amplification and enhanced expression of the c-myc oncogene in mouse SEWA tumour cells.
    Nature. 1985 May 23-29;315(6017):345-7 PMID: 3889656
  9. Activation of the c-myc oncogene by the immunoglobulin heavy-chain gene enhancer after multiple switch region-mediated chromosome rearrangements in a murine plasmacytoma.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3746-50 PMID: 2987943
  10. Microinjected c-myc as a competence factor.
    Science. 1985 Jun 14;228(4705):1313-5 PMID: 4001943
  11. Variant (6;15) translocations in murine plasmacytomas involve a chromosome 15 locus at least 72 kb from the c-myc oncogene.
    EMBO J. 1985 Mar;4(3):675-81 PMID: 3924592
  12. Chromosome translocations clustered 5' of the murine c-myc gene qualitatively affect promoter usage: implications for the site of normal c-myc regulation.
    EMBO J. 1985 Jun;4(6):1441-7 PMID: 2992931
  13. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits.
    J Exp Med. 1970 Mar 1;131(3):515-41 PMID: 4189836
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Growth of factor-dependent hemopoietic precursor cell lines.
    J Exp Med. 1980 Oct 1;152(4):1036-47 PMID: 6968334
  17. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  18. Organization and reorganization of immunoglobulin genes in A-MULV-transformed cells: rearrangement of heavy but not light chain genes.
    Cell. 1981 Dec;27(2 Pt 1):381-90 PMID: 6277505
  19. Active chromatin.
    Nature. 1982 May 27;297(5864):289-95 PMID: 6210847
  20. Functional role for c-myc in mitogenic response to platelet-derived growth factor.
    Nature. 1984 Aug 23-29;310(5979):655-60 PMID: 6088986
  21. DNA sequence associated with chromosome translocations in mouse plasmacytomas.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6622-6 PMID: 6959142
  22. Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas.
    Cell. 1982 Dec;31(2 Pt 1):443-52 PMID: 6819085
  23. Specific chromosomal translocations and the genesis of B-cell-derived tumors in mice and men.
    Cell. 1983 Feb;32(2):311-5 PMID: 6402307
  24. Immunoglobulin gene expression in transformed lymphoid cells.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):825-9 PMID: 6402777
  25. Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):519-23 PMID: 6188153
  26. Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma.
    Proc Natl Acad Sci U S A. 1983 Mar;80(6):1707-11 PMID: 6300869
  27. Cellular myc oncogene is altered by chromosome translocation to an immunoglobulin locus in murine plasmacytomas and is rearranged similarly in human Burkitt lymphomas.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1982-6 PMID: 6572957
  28. A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin.
    Cell. 1983 Apr;32(4):1191-203 PMID: 6301683
  29. Translocation, breakage and truncated transcripts of c-myc oncogene in murine plasmacytomas.
    Nature. 1983 Jun 2-8;303(5916):401-6 PMID: 6304522
  30. Nucleotide sequence of cloned cDNA of human c-myc oncogene.
    Nature. 1983 Jun 23-29;303(5919):725-8 PMID: 6304538
  31. Two promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver.
    Cell. 1983 Jun;33(2):501-8 PMID: 6190572
  32. Consequences of myc invasion of immunoglobulin loci: facts and speculation.
    Cell. 1983 Jul;33(3):647-9 PMID: 6871989
  33. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
    Nature. 1983 Aug 18-24;304(5927):596-602 PMID: 6308472
  34. The human c-myc oncogene: structural consequences of translocation into the IgH locus in Burkitt lymphoma.
    Cell. 1983 Oct;34(3):779-87 PMID: 6414718
  35. Translocations among antibody genes in human cancer.
    Science. 1983 Nov 18;222(4625):765-71 PMID: 6356357
  36. Cellular oncogenes and multistep carcinogenesis.
    Science. 1983 Nov 18;222(4625):771-8 PMID: 6356358
  37. Amplification and expression of the c-myc oncogene in human lung cancer cell lines.
    Nature. 1983 Nov 10-16;306(5939):194-6 PMID: 6646201
  38. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
  39. High resolution banding analysis of the involvement of strain BALB/c- and AKR-derived chromosomes No. 15 in plasmacytoma-specific translocations.
    J Exp Med. 1984 Jan 1;159(1):276-91 PMID: 6607313
  40. Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 PMID: 6320177
  41. Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours.
    EMBO J. 1983;2(12):2375-83 PMID: 6321164
  42. Products of a reciprocal chromosome translocation involving the c-myc gene in a murine plasmacytoma.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):829-33 PMID: 6322170
  43. Translocation of the myc cellular oncogene to the immunoglobulin heavy chain locus in murine plasmacytomas is an imprecise reciprocal exchange.
    Cell. 1984 Apr;36(4):973-82 PMID: 6323031
  44. Mapping of DNase I-hypersensitive sites in the upstream DNA of human embryonic epsilon-globin gene in K562 leukemia cells.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2718-22 PMID: 6232614
  45. Murine T lymphomas in which the cellular myc oncogene has been activated by retroviral insertion.
    Cell. 1984 May;37(1):113-22 PMID: 6327047
  46. Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma.
    Cell. 1984 Jun;37(2):381-91 PMID: 6327064
  47. Transcriptional activation of the translocated c-myc oncogene in mouse plasmacytomas: similar RNA levels in tumor and proliferating normal cells.
    Cell. 1984 Jun;37(2):521-8 PMID: 6327072
  48. Chromosomal translocations activating myc sequences and transduction of v-abl are critical events in the rapid induction of plasmacytomas by pristane and abelson virus.
    J Exp Med. 1984 Jun 1;159(6):1762-77 PMID: 6202819
  49. Effect of somatic mutation within translocated c-myc genes in Burkitt's lymphoma.
    Nature. 1984 Jun 14-20;309(5969):592-7 PMID: 6547209
  50. Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation.
    Science. 1984 Aug 17;225(4663):687-93 PMID: 6431612
  51. Activation of a translocated c-myc gene: role of structural alterations in the upstream region.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6798-802 PMID: 6593728
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3195-202
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554642
Subset
IM
Grants
NIAID NIH HHS · AI00416 · United States
NCI NIH HHS · CA36246 · United States
External Links
PubMed source PMC Full Text View journal details
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