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PMID: 8917507 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 23 ·1996-11-12 ·Pages 12845-50

Yang WM, Inouye C, Zeng Y, Bearss D, Seto E

Abstract

YY1 is a mammalian zinc-finger transcription factor with unusual structural and functional features. It has been implicated as a positive and a negative regulatory factor that binds to the CCATNTT consensus DNA element located in promoters of many cellular and viral genes. A mammalian cDNA that encodes a YY1-binding protein and possesses sequence homology with the yeast transcriptional factor RPD3 has been identified. A Gal4 DNA binding domain-mammalian RPD3 fusion protein strongly represses transcription from a promoter containing Gal4 binding sites. Association between YY1 and mammalian RPD3 requires a glycine-rich region on YY1. Mutations in this region abolish the interaction with mammalian RPD3 and eliminate transcriptional repression by YY1. These data suggest that YY1 negatively regulates transcription by tethering RPD3 to DNA as a cofactor and that this transcriptional mechanism is highly conserved from yeast to human.

MeSH Terms
Amino Acid Sequence Animals DNA-Binding Proteins/biosynthesis,metabolism Erythroid-Specific DNA-Binding Factors Fungal Proteins/biosynthesis,chemistry,metabolism Glutathione Transferase/biosynthesis Histone Deacetylases Humans Mammals Mice Molecular Sequence Data Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis,chemistry,metabolism Recombinant Proteins/biosynthesis,metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Transcription Factors/biosynthesis,chemistry,metabolism Transcription, Genetic YY1 Transcription Factor Zinc Fingers
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Fungal Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Recombinant Proteins Saccharomyces cerevisiae Proteins Transcription Factors YY1 Transcription Factor YY1 protein, human Yy1 protein, mouse Glutathione Transferase RPD3 protein, S cerevisiae Histone Deacetylases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yang W M
Moffitt Cancer Center and Research Institute, University of South Florida, Tampa 33612, USA.
Inouye C
Zeng Y
Bearss D
Seto E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.23.12845
Published
1996-11-12
Pages
12845-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24008
Subset
IM
Databases
GENBANK
U31758, U31814, X78454
External Links
PubMed source DOI: 10.1073/pnas.93.23.12845 PMC Full Text View journal details
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