Home LiteratureArticle Details
PMID: 9060439 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Aspergillus nidulans maintains short telomeres throughout development.

Nucleic acids research ·Vol. 25 ·No. 7 ·1997-04-01 ·Pages 1426-31

Bhattacharyya A, Blackburn EH

Abstract

We report the identification and cloning of the telomeres of the filamentous fungus,Aspergillus nidulans. We have identified three classes of cloned chromosomal ends based on the telomere-associated sequences (TASs) and demonstrated that the telomeric repeat sequence is TTAGGG, identical to that found in vertebrates, including humans, and some lower eukaryotes. One category of telomere clones was found to contain internal, variant TAAGGG repeats. The A.nidulans telomeric tract length is strikingly short (4-22 repeats). We demonstrate that telomere length is remarkably stable in different cell types and at altered growth temperatures, suggesting a highly regulated mechanism for length control.

MeSH Terms
Aspergillus nidulans/growth & development Base Sequence Blotting, Southern Cloning, Molecular DNA, Fungal/chemistry Endodeoxyribonucleases/metabolism Molecular Sequence Data Sequence Analysis, DNA Telomere/genetics Temperature
Chemicals
DNA, Fungal Endodeoxyribonucleases exonuclease Bal 31
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhattacharyya A
Department of Microbiology and Immunology, University of California, San Francisco, CA 94143-0414, USA. anamitra@cgl.ucsf.edu
Blackburn E H
References (22)
22 references, click to expand
  1. The Stability of Broken Ends of Chromosomes in Zea Mays.
    Genetics. 1941 Mar;26(2):234-82 PMID: 17247004
  2. Characterization of Arabidopsis thaliana telomeres isolated in yeast.
    Nucleic Acids Res. 1992 Aug 11;20(15):4039-46 PMID: 1508688
  3. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  4. Mitotic regulation in Aspergillus nidulans.
    Ciba Found Symp. 1992;170:115-23; discussion 123-9 PMID: 1483343
  5. Unusually large telomeric repeats in the yeast Candida albicans.
    Mol Cell Biol. 1993 Jan;13(1):551-60 PMID: 8417351
  6. Control of telomere growth by interactions of RAP1 with the most distal telomeric repeats.
    Nature. 1996 Sep 26;383(6598):354-7 PMID: 8848051
  7. Telomere-led premeiotic chromosome movement in fission yeast.
    Science. 1994 Apr 8;264(5156):270-3 PMID: 8146661
  8. The Behavior in Successive Nuclear Divisions of a Chromosome Broken at Meiosis.
    Proc Natl Acad Sci U S A. 1939 Aug;25(8):405-16 PMID: 16577924
  9. Yeast telomere repeat sequence (TRS) improves circular plasmid segregation, and TRS plasmid segregation involves the RAP1 gene product.
    Mol Cell Biol. 1992 May;12(5):1997-2009 PMID: 1569937
  10. Preparation of large molecular weight DNA from the fungus Aspergillus nidulans.
    J Gen Microbiol. 1978 Jun;106(2):387-9 PMID: 353226
  11. Structure and polymorphism of human telomere-associated DNA.
    Cell. 1990 Oct 5;63(1):119-32 PMID: 2208276
  12. An unusual sequence arrangement in the telomeres of the germ-line micronucleus in Tetrahymena thermophila.
    Genes Dev. 1995 Jan 1;9(1):59-71 PMID: 7828852
  13. Structure of the growing telomeres of Trypanosomes.
    Cell. 1984 Feb;36(2):459-68 PMID: 6319026
  14. Genetic engineering of filamentous fungi.
    Science. 1989 Jun 16;244(4910):1313-7 PMID: 2525275
  15. Structure and function of telomeres.
    Annu Rev Genet. 1989;23:579-604 PMID: 2694944
  16. Identification of a telomeric fragment from the right arm of chromosome III of Aspergillus nidulans.
    FEMS Microbiol Lett. 1991 May 15;64(2-3):295-7 PMID: 1884987
  17. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
    Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
  18. Sequencing of Saccharomyces telomeres cloned using T4 DNA polymerase reveals two domains.
    Mol Cell Biol. 1990 Aug;10(8):4415-9 PMID: 2196453
  19. Hypervariable ultra-long telomeres in mice.
    Nature. 1990 Sep 27;347(6291):400-2 PMID: 2170845
  20. Identification of developmental regulatory genes in Aspergillus nidulans by overexpression.
    Genetics. 1995 Feb;139(2):537-47 PMID: 7713416
  21. Conservation of the human telomere sequence (TTAGGG)n among vertebrates.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7049-53 PMID: 2780561
  22. A conserved sequence motif within the exceptionally diverse telomeric sequences of budding yeasts.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3453-7 PMID: 8159768
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.7.1426
Email
anamitra@cgl.ucsf.edu
Published
1997-04-01
Pages
1426-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146599
Subset
IM
Grants
NIGMS NIH HHS · GM 26259 · United States
Databases
GENBANK
U95210, U95211, U95212
External Links
PubMed source DOI: 10.1093/nar/25.7.1426 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2