Home LiteratureArticle Details
PMID: 8417351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Unusually large telomeric repeats in the yeast Candida albicans.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 551-60

McEachern MJ, Hicks JB

Abstract

We have identified sequences at the telomeres of the yeast Candida albicans and have found that they are composed of tandem copies of a 23-bp sequence. Through the cloning of native telomeric ends and the characterization and cloning of a "healed" end, we demonstrate that these repeated sequences are sufficient to function as a telomere. All copies of the 23-bp repeat that have been sequenced from a number of C. albicans strains are identical. In contrast, adjacent subtelomeric sequences are variable both between strains and within the WO-1 strain. In the WO-1 strain, the lengths of the telomeres are dependent upon growth temperature and are substantially longer at higher temperatures. Telomere growth is accompanied by increases in the number of the 23-bp repeats present on the telomeric fragments. These results suggest that either telomerase-maintained telomeres can be more complex in structure than was previously imagined or that Candida telomeres are maintained via a telomerase-independent mechanism.

MeSH Terms
Base Sequence Candida albicans/genetics,growth & development Chromosomes, Fungal/ultrastructure Cloning, Molecular DNA, Fungal/genetics Molecular Sequence Data Repetitive Sequences, Nucleic Acid Restriction Mapping Telomere Temperature
Chemicals
DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McEachern M J
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Hicks J B
References (44)
44 references, click to expand
  1. Expression of the RESA gene in Plasmodium falciparum isolate FCR3 is prevented by a subtelomeric deletion.
    Mol Cell Biol. 1989 Aug;9(8):3584-7 PMID: 2677678
  2. Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification.
    J Bacteriol. 1991 Jan;173(2):842-50 PMID: 1987167
  3. The Stability of Broken Ends of Chromosomes in Zea Mays.
    Genetics. 1941 Mar;26(2):234-82 PMID: 17247004
  4. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  5. Recognition of a chromosome truncation site associated with alpha-thalassaemia by human telomerase.
    Nature. 1991 Oct 3;353(6343):454-6 PMID: 1896089
  6. Generation of telomere-length heterogeneity in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):534-8 PMID: 3277178
  7. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3015-9 PMID: 6265931
  8. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs.
    Nature. 1990 Mar 8;344(6262):126-32 PMID: 1689810
  9. Developmentally programmed healing of chromosomes by telomerase in Tetrahymena.
    Cell. 1991 Nov 15;67(4):823-32 PMID: 1934071
  10. Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs.
    Cell. 1987 Dec 24;51(6):899-908 PMID: 3690664
  11. Structure and function of telomeres.
    Nature. 1991 Apr 18;350(6319):569-73 PMID: 1708110
  12. "White-opaque transition": a second high-frequency switching system in Candida albicans.
    J Bacteriol. 1987 Jan;169(1):189-97 PMID: 3539914
  13. Mitochondrial telomeres: surprising diversity of repeated telomeric DNA sequences among six species of Tetrahymena.
    Cell. 1988 Feb 12;52(3):367-74 PMID: 3125982
  14. Healing of broken linear dicentric chromosomes in yeast.
    Genetics. 1984 Feb;106(2):207-26 PMID: 6365688
  15. Growth of chromosome ends in multiplying trypanosomes.
    Nature. 1983 Jun 16-22;303(5918):592-7 PMID: 6304531
  16. A truncated human chromosome 16 associated with alpha thalassaemia is stabilized by addition of telomeric repeat (TTAGGG)n.
    Nature. 1990 Aug 30;346(6287):868-71 PMID: 1975428
  17. An overhanging 3' terminus is a conserved feature of telomeres.
    Mol Cell Biol. 1989 Jan;9(1):345-8 PMID: 2927395
  18. Recombination occurs during telomere formation in yeast.
    Nature. 1989 Feb 2;337(6206):429-33 PMID: 2536898
  19. Differential size variations between transcriptionally active and inactive telomeres of Trypanosoma brucei.
    Nucleic Acids Res. 1983 Dec 10;11(23):8137-47 PMID: 6324074
  20. Variations of Candida albicans electrophoretic karyotypes.
    J Bacteriol. 1991 Oct;173(20):6586-96 PMID: 1917880
  21. Developmentally regulated telomere addition in Tetrahymena thermophila.
    Nucleic Acids Res. 1988 Jun 24;16(12):5569-85 PMID: 2838822
  22. Telomerase primer specificity and chromosome healing.
    Nature. 1991 Oct 3;353(6343):451-4 PMID: 1896088
  23. Development of autonomously replicating plasmids for Candida albicans.
    Mol Cell Biol. 1987 Jan;7(1):209-17 PMID: 3031460
  24. Structure of the growing telomeres of Trypanosomes.
    Cell. 1984 Feb;36(2):459-68 PMID: 6319026
  25. Dynamics of telomere length variation in Tetrahymena thermophila.
    Cell. 1987 Jul 31;50(3):477-83 PMID: 3607876
  26. Structure and function of telomeres.
    Annu Rev Genet. 1989;23:579-604 PMID: 2694944
  27. A Candida albicans dispersed, repeated gene family and its epidemiologic applications.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1452-6 PMID: 3278313
  28. In vivo construction of linear vectors based on killer plasmids from Kluyveromyces lactis: selection of a nuclear gene results in attachment of telomeres.
    Mol Cell Biol. 1989 Sep;9(9):3931-7 PMID: 2779572
  29. Dosage of the smallest chromosome affects both the yeast-hyphal transition and the white-opaque transition of Candida albicans WO-1.
    J Bacteriol. 1991 Dec;173(23):7436-42 PMID: 1938940
  30. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis.
    Nature. 1989 Jan 26;337(6205):331-7 PMID: 2463488
  31. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats.
    Cell. 1989 Nov 3;59(3):521-9 PMID: 2805070
  32. Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe.
    Mol Cell Biol. 1987 Dec;7(12):4424-30 PMID: 2830493
  33. Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules.
    Nucleic Acids Res. 1988 Jul 25;16(14B):6953-72 PMID: 3136437
  34. Telomeres: do the ends justify the means?
    Cell. 1984 May;37(1):7-8 PMID: 6722877
  35. Sequencing of Saccharomyces telomeres cloned using T4 DNA polymerase reveals two domains.
    Mol Cell Biol. 1990 Aug;10(8):4415-9 PMID: 2196453
  36. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.
    Cell. 1985 Dec;43(2 Pt 1):405-13 PMID: 3907856
  37. Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
    Nature. 1988 Apr 14;332(6165):656-9 PMID: 2833706
  38. Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.
    Nucleic Acids Res. 1987 Feb 25;15(4):1717-28 PMID: 3029725
  39. A G-protein alpha subunit from asexual Candida albicans functions in the mating signal transduction pathway of Saccharomyces cerevisiae and is regulated by the a1-alpha 2 repressor.
    Mol Cell Biol. 1992 May;12(5):1977-85 PMID: 1569935
  40. Functional evidence for an RNA template in telomerase.
    Science. 1990 Feb 2;247(4942):546-52 PMID: 1689074
  41. Developmentally controlled telomere addition in wild-type and mutant paramecia.
    Mol Cell Biol. 1988 Jan;8(1):251-8 PMID: 3336360
  42. Telomere reduction in human colorectal carcinoma and with ageing.
    Nature. 1990 Aug 30;346(6287):866-8 PMID: 2392154
  43. DNA content, kinetic complexity, and the ploidy question in Candida albicans.
    Mol Cell Biol. 1982 Jul;2(7):853-62 PMID: 6765567
  44. Large deletions result from breakage and healing of P. falciparum chromosomes.
    Cell. 1988 Dec 2;55(5):869-74 PMID: 3056622
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.551-560.1993
Published
1993-01-00
Pages
551-60
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358934
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.13.1.551-560.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2