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PMID: 9034336 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fission yeast pheromone blocks S-phase by inhibiting the G1 cyclin B-p34cdc2 kinase.

The EMBO journal ·Vol. 16 ·No. 3 ·1997-02-03 ·Pages 534-44

Stern B, Nurse P

Abstract

Yeast pheromones block cell cycle progression in G1 in order to prepare mating partners for conjugation. We have investigated the mechanism underlying pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe. We find that the G1-specific transcription factor p65cdc10-p72res1/sct1 which controls the expression of S-phase genes is fully activated in pheromone, unlike the analogous control in budding yeast. In contrast, the G1 function of p34cdc2 acting after activation of the G1-specific transcription is blocked. Pheromone inhibits the p34cdc2 kinase associated with both the G1-specific B-type cyclin p45cig2 and the B-type cyclin p56cdc13 and overexpression of p45cig2 or p47cdc13delta90 overcomes the pheromone-induced G1 arrest. G1 arrest is compromised in enlarged cells. We suggest that onset of S-phase is controlled by pheromone inhibiting the B-cyclin-associated kinase in G1, and that increasing cell size contributes to the mechanism for pheromone adaptation. Thus, pheromone in fission and budding yeast acts similarly in inhibiting the G1 cyclin-dependent kinase (CDK), but differs in its effects on the G1/S transcriptional control, suggesting that inhibition of CDKs may be a more general mechanism for the control of G1 progression compared with G1/S transcriptional control.

MeSH Terms
Blotting, Northern CDC2 Protein Kinase/metabolism Cell Count/drug effects Cell Cycle/drug effects,physiology Cell Size/drug effects Cyclin B Cyclin-Dependent Kinases/antagonists & inhibitors,metabolism Cyclins/metabolism DNA/analysis Enzyme Inhibitors/pharmacology Flow Cytometry Fungal Proteins/metabolism Gene Expression Regulation, Fungal/genetics Hydroxyurea/pharmacology Pheromones/pharmacology Protein Sorting Signals/pharmacology S Phase/drug effects,genetics Schizosaccharomyces/metabolism Schizosaccharomyces pombe Proteins Temperature Transcription Factors/metabolism,pharmacology
Chemicals
Cyclin B Cyclins Enzyme Inhibitors Fungal Proteins Pheromones Protein Sorting Signals Schizosaccharomyces pombe Proteins Transcription Factors map2 protein, S pombe DNA CDC2 Protein Kinase Cyclin-Dependent Kinases Hydroxyurea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stern B
Cell Cycle Laboratory, Imperial Cancer Research Fund, London, UK.
Nurse P
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48 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.3.534
Published
1997-02-03
Pages
534-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1169657
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.3.534 PMC Full Text View journal details
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