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PMID: 8631306 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins.

The EMBO journal ·Vol. 15 ·No. 4 ·1996-02-15 ·Pages 850-60

Fisher DL, Nurse P

Abstract

Deletion of the fission yeast mitotic B-type cyclin gene cdc13 causes cells to undergo successive rounds of DNA replication. We have used a strain which expresses cdc13 conditionally to investigate re-replication. Activity of Start genes cdc2 and cdc10 is necessary and p34cdc2 kinase is active in re-replicating cells. We tested to see whether other cyclins were required for re-replication using cdc13delta. Further deletion of cig1 and puc1 had no effect, but deletion of cig2/cyc17 caused a severe delay in re-replication. Deletion of cig1 and cig2/cyc17 together abolished re-replication completely and cells arrested in G1. This, and analysis of the temperature sensitive cdc13-117 mutant, suggests that cdc13 can effectively substitute for the G1 cyclin activity of cig2/cyc17. We have characterized p56cdc13 activity and find evidence that in the absence of G1 cyclins, S-phase is delayed until the mitotic p34cdc2-p56cdc13 kinase is sufficiently active. These data suggest that a single oscillation of p34cdc2 kinase activity provided by a single B-type cyclin can promote ordered progression into both DNA replication and mitosis, and that the level of cyclin-dependent kinase activity may act as a master regulator dictating whether cells undergo S-phase or mitosis.

MeSH Terms
CDC2 Protein Kinase/physiology Cell Cycle Cell Cycle Proteins/physiology Cyclin B Cyclins/metabolism,physiology DNA Replication Enzyme Activation Fungal Proteins/physiology Mitosis S Phase Schizosaccharomyces/cytology,enzymology Schizosaccharomyces pombe Proteins Transcription Factors
Chemicals
Cell Cycle Proteins Cig2 protein, S pombe Cyclin B Cyclins Fungal Proteins Schizosaccharomyces pombe Proteins Transcription Factors cdc10 protein, S pombe CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fisher D L
Cell Cycle Laboratory, Imperial Cancer Research Fund, London, UK.
Nurse P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-02-15
Pages
850-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450283
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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