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PMID: 9015302 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF).

The Journal of cell biology ·Vol. 136 ·No. 2 ·1997-01-27 ·Pages 307-17

Mayer A, Wickner W

Abstract

Vacuole inheritance in yeast involves the formation of tubular and vesicular "segregation structures" which migrate into the bud and fuse there to establish the daughter cell vacuole. Vacuole fusion has been reconstituted in vitro and may be used as a model for an NSF-dependent reaction of priming, docking, and fusion. We have developed biochemical and microscopic assays for the docking step of in vitro vacuole fusion and characterized its requirements. The vacuoles must be primed for docking by the action of Sec17p (alpha-SNAP) and Sec18p (NSF). Priming is necessary for both fusion partners. It produces a labile state which requires rapid docking in order to lead productively to fusion. In addition to Sec17p/Sec18p, docking requires the activity of the Ras-like GTPase Ypt7p. Unlike Sec17p/Sec18p, which must act before docking, Ypt7p is directly involved in the docking process itself.

MeSH Terms
Adenosine Triphosphatases Adenosine Triphosphate/metabolism Carrier Proteins/metabolism Cell Fractionation Cytosol/metabolism Dimerization Fungal Proteins/metabolism GTP Phosphohydrolases/metabolism GTP-Binding Proteins/metabolism Kinetics Membrane Fusion Membrane Proteins/metabolism Microscopy Mutation Repressor Proteins Saccharomyces cerevisiae/metabolism,ultrastructure Saccharomyces cerevisiae Proteins Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Temperature Thioredoxins/metabolism Vacuoles/metabolism Vesicular Transport Proteins rab GTP-Binding Proteins
Chemicals
Carrier Proteins Fungal Proteins Membrane Proteins PBI2 protein, S cerevisiae Repressor Proteins SEC17 protein, S cerevisiae Saccharomyces cerevisiae Proteins Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Vesicular Transport Proteins Thioredoxins Adenosine Triphosphate Adenosine Triphosphatases GTP Phosphohydrolases GTP-Binding Proteins SEC18 protein, S cerevisiae YPT7 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayer A
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755-3844, USA.
Wickner W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.2.307
Published
1997-01-27
Pages
307-17
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2134819
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.136.2.307 PMC Full Text View journal details
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