Enzymatic and immunological characterization of a new cephalosporinase from Enterobacter aerogenes.

Antimicrobial agents and chemotherapy ·Vol. 12 ·No. 2 ·1977-08-00 ·Pages 201-5

Letarte R, Devaud-Felix M, Pechere JC, Allard-Leprohon D

Abstract

A hospital strain of Enterobacter aerogenes (MULB 250) isolated from a urinary tract infection was found to be cephalosporin and ampicillin resistant and carbenicillin susceptible. The beta-lactamase produced by this strain was extracted and purified by means of affinity chromatography, using a cephalosporin C-bound Sepharose 4B column. The purified enzyme was tested for hydrolysis of penicillin and various cephalosporins. The K(m) value is 11.8 muM for benzyl penicillin and 130 muM for cephalosporin C. The isoelectric point of the enzyme is 9.3, and its molecular weight is 29,500 +/- 1,000. Rabbit antiserum obtained against this MULB 250 beta-lactamase showed no cross-reaction with other penicillinases or cephalosporinases in neutralization tests. Comparisons of results obtained with other beta-lactamases, particularly from Enterobacter cloacae P99, indicate that the Enterobacter MULB 250 enzyme presents a typical cephalosporinase profile. As far as we know, this type of enzyme is relatively rare.

MeSH Terms

Amidohydrolases/isolation & purification Antibody Specificity Cephalosporinase/immunology,isolation & purification,metabolism Chromatography, Affinity Drug Resistance, Microbial Enterobacter/enzymology Enterobacteriaceae/enzymology Isoelectric Focusing Kinetics Molecular Weight

Chemicals

Amidohydrolases Cephalosporinase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Letarte R

Devaud-Felix M

Pechere J C

Allard-Leprohon D

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25 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.12.2.201

Published

1977-08-00

Pages

201-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC429885

Subset

External Links

PubMed source DOI: 10.1128/AAC.12.2.201 PMC Full Text View journal details

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Enzymatic and immunological characterization of a new cephalosporinase from Enterobacter aerogenes.

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