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PMID: 9006011 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Virulence and arsenic resistance in Yersiniae.

Journal of bacteriology ·Vol. 179 ·No. 3 ·1997-02-00 ·Pages 612-9

Neyt C, Iriarte M, Thi VH, Cornelis GR

Abstract

The genus Yersinia contains three pathogenic species: Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica. Only a few biotypes and serotypes of Y. enterocolitica are pathogenic, and these form two distinct groups: some are of low virulence, and they are encountered worldwide; others, mainly encountered in North America, are markedly more virulent. All pathogenic yersiniae possess a 70-kb virulence plasmid called pYV which encodes secreted antihost proteins called Yops as well as a type III secretion machinery that is required for Yop secretion. Genes encoding Yop synthesis and secretion are tightly clustered in three quadrants of the pYV plasmid. We show here that in the low-virulence strains of Y. enterocolitica, the fourth quadrant of the plasmid contains a new class II transposon, Tn2502. This transposon encodes a defective transposase, but transposition can be complemented in trans by Tn2501, another class II transposon. Tn2502 was not detected in the pYV plasmids of the more virulent American strains of Y. enterocolitica, of Y. pseudotuberculosis, and of Y. pestis. Tn2502 confers arsenite and arsenate resistance. This resistance involves four genes; three are homologous to the arsRBC genes present on the Escherichia coli chromosome, but no homolog of the fourth one, arsH, has been found. The systematic presence of such a resistance operon on a virulence plasmid is unusual and could be related to the recent spread of low-virulence Y. enterocolitica strains. The presence of this ars operon also constitutes the first significant difference between the pYV plasmids from different Yersinia species.

MeSH Terms
Amino Acid Sequence Arsenates/pharmacology Arsenic/pharmacology Arsenicals/pharmacology Arsenites/pharmacology Bacterial Outer Membrane Proteins/genetics Bacterial Proteins DNA Transposable Elements/genetics Drug Resistance, Microbial Escherichia coli Proteins Genes, Bacterial Molecular Sequence Data Operon Regulon Sequence Homology, Amino Acid Species Specificity Trans-Activators/genetics Yersinia/drug effects,pathogenicity Yersinia enterocolitica/genetics Yersinia pseudotuberculosis/genetics
Chemicals
ArsR protein, E coli Arsenates Arsenicals Arsenites Bacterial Outer Membrane Proteins Bacterial Proteins DNA Transposable Elements Escherichia coli Proteins Trans-Activators Arsenic
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Neyt C
Microbial Pathogenesis Unit, International Institute of Cellular and Molecular Pathology, and Faculté de Médecine, Université Catholique deLouvain, Brussels, Belgium.
Iriarte M
Thi V H
Cornelis G R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.3.612-619.1997
Published
1997-02-00
Pages
612-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178738
Subset
IM
Databases
GENBANK
U58366
External Links
PubMed source DOI: 10.1128/jb.179.3.612-619.1997 PMC Full Text View journal details
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